We narrowed to 161 results for: mPA-GFP

...plasmids described in this work including the soCoChR-GFP plasmid as well as several derivatives encoding alternative...ShadowG, a novel dark green fluorescent protein (GFP) with an absorption peak of 486 nm and ShadowY, a...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust...a 1.2-fold greater extinction coefficient when compared to sREACH. Find the FLIM-FRET acceptors here. ...

...this method through monitoring GFP degradation, this protein compartment was used to optimize D-glucaric... hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun Luo’s lab expresses membrane tethered GFP to enhance...a single enzyme. Compared with previous research, which required protein compartments to co-localize multiple...targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng's lab... antibodies for aminoacyl tRNA synthetases Nanocompartments for yeast bioproduction pathways The CRISPR...Research Center developed a pooled plasmid library encompassing all possible amino acid mutations of the receptor...mutations which do not interfere with ACE2 binding, but impact recognition by specific anti-SARS-CoV-2 antibodies...

...extent they overlap. Figure 4 compares the spectra of enhanced GFP (eGFP) and FITC, the latter being a...to fuse them with a fluorescent reporter, such as GFP or mCherry, through genetic modification. As your... you can also use fluorescent reporters, such as GFP, and fluorophore-conjugated antibodies together in...vector also encodes for eGFP, which serves as a transduction reporter. Note that eGFP is not fused to CD45...-Cy7. Next comes our transduction reporter, eGFP. As eGFP emits fluorescence by itself and does not need...cytometer which channel to use to detect eGFP. As you saw in Figure 4, eGFP strongly overlaps with FITC, hence...results in a higher-wavelength emission spectrum compared to APC. This happens due to a process called Fluorescence...

... FRET tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools...few new ones that are not used so much but have comparable expression levels to things people do use a lot...committees, and how the culture of the university impacts the number of women reaching senior positions. ... those amazing students that want to have a big impact on the world and tell them to work in plant science...

... cassette with an ubiquitous promoter to express GFP in a group of young rats. We found that, in this ...label norepinephrine (NE) expressing neurons and GFP (green) to label neurons transduced by each serotype...much the spread of the virus to adjacent areas impacted the experiments we wanted to perform. It required...literature, scientists can use the Data Hub to compare different methods and vectors, and use this data... rat CNS: AAV PHP.EB vector dose–response and comparison to AAV PHP.B. Gene Therapy, 25(5), 392–400. https..., C., & Flotte, T. R. (2015). Stability and compatibility of recombinant adeno-associated virus under ...

...GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato...fluorescent tools for labeling structures and compartments in mammalian cells By Angela Abitua Genetically...are often used to label  specific structures, compartments, or specific localization of biomolecules within...usually much easier to clone, express, and purify compared to antibodies. Nanobodies are easily expressed...pMAK463 (NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) The pMAK461-64 plasmids were...introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator...

...two photons of red light are needed to excite GFP compared to only one of blue. Only at the focal point...red light instead of blue to excite a molecule of GFP. Because red light has approximately half the energy...infinitely small point of light – think of a single GFP molecule – will appear as a fuzzy 200-300 nm spot...

...mouse brains expressing green fluorescent protein (GFP) (6).   The Dodt and Frank Bradke laboratories later...subpopulation of neurons are brightly labeled with GFP (e.g. Thy1-YFP-H mice).  However, we recently developed... nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical...labeling in whole tissue (15).  CLARITY is also compatible with LSFM, a method termed COLM (clarity-optimized...spatiotemporal control over labeling methods as compared to transgenic animals (17).  These viral plasmids...

...protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes... I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein Guide Expression Vectors from...into a translated protein. As depicted in the accompanying cartoon, they have a multitude of uses including...ProtA IgG-binding domains. TAP has since come to encompass several other tag combinations, though frequently...

... N. C. A., et al. "Evaluation and comparison of the GUS, LUC and GFP reporter system for gene expression...tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g. GFP) can be used in many ways. Often a FP is fused to...

...Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters...Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. C) Quantifications...-multi (Walton et al. 2024). This platform is compatible with enrichment, single-cell sequencing, and ...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...scAAV). However, scAAV vectors have limited size compared to single-strand AAV (ssAAV). To maintain the ...

...P2A-linked accessory genes gvpNJKFGWV marked with Emerald GFP. These plasmids can be used directly in transient...gating for the top quartile (or higher) of BFP and GFP producing cells. After FACS, return the cells to ...structural protein GvpA, which seemed overexpressed compared to accessory proteins. Excess gvpA plasmid added...

...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321...S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid...pSerOTS-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ... prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...

...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...

...biosensors as an example. Many calcium biosensors include GFP fused to calmodulin (CaM) and the chicken myosin ...biosensors can be targeted to a particular cellular compartment such as the nucleus, mitochondria, endoplasmic... biosensor: which fluorescent protein is most compatible with your lab and experimental setup? Consider...

...blot showing GFP expression in cells that have been transfected with a plasmid encoding GFP. Actin, a common...production strategy has advantages and disadvantages compared to each other. Polyclonal antibodies These antibodies...stability. Additionally, the choice of vector can impact the expression level of an antibody (Ayyar et al...Single-chain variable fragment (ScFv) Figure 2: Comparison between the IgG antibody and scFv. Because... their lower affinity for their target protein compared to whole antibodies (Crivianu-Gaita 2016). Get...Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies are the...fluorescence. Western blots are frequently used to compare relative levels of protein expression between cell...

...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...encode fluorescent reporters and can be used to compare the tropism of different serotypes. In addition... 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...

...fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight...norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023).     Figure 1: Properties...μm (neurons). Figure and caption adapted from Kagiampaki et al., 2023. nLight nLight sensors are based... https://doi.org/10.1016/j.neuron.2019.02.037 Kagiampaki, Z., Rohner, V., Kiss, C., Curreli, S., Dieter...

...highlighted back in 2015, where sdAbs were used to create GFP scaffolds for transcriptional activation.  sdAbs ...to revisit some of the ways these animals have impacted humans through biomedical research. The Camelid...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...blog over the years and have described how they compare to other antibodies and affinity reagents. But ...And I suspect we will only continue to see their impact grow as new sdAbs become easier to generate and...