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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,020 results for: nts

Showing: 61 - 80 of 1020 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...pseudovirus, where the structural and functional components of one virus (such as HIV, murine leukemia virus...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID...

  2. Fluorescent Proteins: FRET

    Type
    Collection
    ...(dark) acceptor, used for FLIM measurements Note that FRET measurements can be influenced by many factors...Find plasmids and resources for FRET experiments to measure protein interactions or conformation changes...molecules have been demonstrated for use in FRET experiments. FRET efficiency can be measured using fluorescence...Introduction to FRET and Tips for Using FRET in Your Experiments . Find many FRET-based biosensors in Addgene'...

  3. COVID-19 Resources

    Type
    Collection
    ...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Whitehead Libraries of Spike (S) Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep ...Extraction Without A Kit Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Link opens in a new window) COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv (Link opens in a new ...differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome...

  4. CRISPR Plasmids - Tagging

    Type
    Collection
    ...generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled up for...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...More details on how to use this plasmid in your experiments can be found below and in the supplemental materials... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

  5. Rett Syndrome

    Type
    Collection
    ... launched in 2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2...syndrome is a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females...skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast to the sustained...there is wide variability in symptoms even within patients with the same mutation, the location and type ...2007. Correlation between clinical severity in patients with Rett syndrome with a p.R168X or p.T158M MECP2...

  7. The Pleiades Promoter Project

    Type
    Collection
    ...intron-lacZ/NLS Ple144 NTSR1 pEMS1137 EGFP/cre/NLS Ple144 NTSR1 pEMS1394 EGFP/NLS Ple146 NTSR1 pEMS1396 EGFP/NLS.../NLS Ple146 NTSR1 pEMS1617 intron-lacZ/NLS Ple147 NTSR1 pEMS1397 EGFP/NLS Ple148 OLIG1 pEMS1169 EGFP/NLS...

  8. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...systems, allowing for the integration of large fragments of DNA at specific locations. The Cas effector...recruiting the transposase (comprised of multiple components) to target locations in the genome defined by...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

  9. TALEN Engineering

    Type
    Collection
    ...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...assembly. Links to additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...leaders providing research opportunities to their students and other members of the MCC community. They support...courses, at any type of institution, and at different points in their careers to participate in the community...single protein with many available isoforms and mutants provides a shared experience for the research community...PMID: 35921892 (Link opens in a new window) Acknowledgments The MCC and plasmid construction was funded...

  11. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR/Cas system makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, ...the mouse genome is made up of 3 x 109 nucleotides (nt), and encodes 23,000 or so genes. It would be great...the gRNA will seek out its target among the 3 X 109 nt of genetic content in the mouse genome and the Cas9...gRNA, Cas9, and donor oligonucleotide or plasmid components are brought together and microinjected into either...eggs. Or, to avoid handling embryos ex vivo, the components can be electroporated into the oviduct of pregnant...CRISPR is so efficient that you can inject the reagents directly into fertilized mouse eggs, circumventing...friends and colleagues while working with these reagents. Other than that, be nice to your microinjectionist...

  12. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments 124606 SOX2-...87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic...Troponin I, slow skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres...

  13. AAV Packaged on Request

    Type
    Collection
    ...mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity...AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep from Addgene... processes to make sure it is ready for your experiments. Shipping 2–7 days We will email you with tracking...Once you start getting results from your AAV experiments, remember to share your protocols and results...

  14. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...plasmid use 30 nt oligos for cloning but PX330 only uses 20 nt? A16: The reason for the 30 nt region on the...In the pre-processing form, the crRNA contains 30 nt of the target spacer, but then when expressed in ...double nickase for my CRISPR genome engineering experiments? A1: When assessing which nickase type to use...use for your CRISPR genome engineering experiments, consider that wildtype Cas9 with optimized chimeric...efficiency is very high. Q6: How should I design experiments for multiplexed targeting with Zhang lab plasmids...

  15. Viral Production

    Type
    Collection
    ...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

  16. CRISPR References and Information

    Type
    Collection
    ... pipeline for the analysis of genome editing experiments. It enables rapid and intuitive interpretation... for the purpose of designing and evaluating experiments. Developed by the Hyongbum Kim Lab . Green Listed... range of reference genomes, including animals, plants, bacteria, fungi, protists, and viruses. Developed...

  17. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... kb ~3.9kb crRNA/gRNA length gRNA: ~100 nt crNA: ~42 nt Requires tracrRNA? Yes No dsDNA cleavage...Cas9 and Cpf1 CRISPR Nucleases   Cas9 Cpf1 Components crRNAtracrRNACas9 crRNACpf1 Endonuclease...AAV transduction.  In most of Zetsche et al’s experiments, a single vector co-expressing Cpf1 and a crRNA...culture of mouse neurons and in vivo. For these experiments, cells were infected with a 1:1 ratio of two ...editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple and effective...thoughts about Cpf1 multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex...

  18. Deisseroth INTRSECT Collection

    Type
    Collection
    ...these ORF fragments (which have become exons) determines the logical expression requirements of Cre and...References The development of powerful molecular reagents for biology have propelled our understanding of... for the molecular design and testing of novel INTSRECT plasmids for groups interested in producing their...

  19. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs ...screened for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more...keep the following in mind: CRISPR screening experiments require electroporation to amplify the pooled...confirm library representation. CRISPR screening experiments require large quantities of cells and therefore...

  20. Antibodies

    Type
    Collection
    ...recombinant antibody collection. These affinity reagents are expressed and purified from plasmids in our...research. We are building a collection of affinity reagents that are expressed from plasmids. These antibodies...therefore makes the sequences of these affinity reagents available to scientists. Additionally, we have...
Showing: 61 - 80 of 1020 results