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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,023 results for: nts

Showing: 61 - 80 of 1023 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Empty backbones are plasmids containing only the elements needed for replication in the host cell and are...choosing what plasmid backbone to use, you have many elements to consider. Here is a guide to a selection of... 3x HA tag fusion with your gene of interest in plants Myc Epitope tag pKMyc - N-terminal Myc tag for ...-nosT - Transient expression vector for eGFP in plants Find FP tagging vectors in budding yeast pHT101... transient expression is sufficient for some experiments, scientists often want to create stable ...species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids and Resources collection... miRNA and 3' UTR mRNA regulation pIS0 - 3'UTR segments of target genes can be inserted into this firefly...

  2. Neurodegeneration Research Collection

    Type
    Collection
    ...for many years and lead to a major burden on the patients, their families and caretakers, and the health...general. The required care for these millions of patients results in billions of dollars spent each year...changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking...after a person's mid-sixties. The early-onset form accounts for less than 10 percent of all cases of Alzheimer's...plasmids expressing wild type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative...discovery and advance development of diagnostics and treatments for Alzheimer’s disease and related disorders...foundation offers curated information on tools and reagents (Link opens in a new window) such as huntingtin...

  3. Resolute Plasmid Collection

    Type
    Collection
    ...The RESOLUTE mission is to develop data and reagents to boost the research focused on Solute carrier ...Union. The RESOLUTE mission is to develop data and reagents to boost the research focused on SLCs. Through...-access ethos’ to the results, techniques, and reagents with the highest-possible quality of research ...knowledgebase that features, among others, genetic variants and disease associations. Plasmids This plasmid...libraries are useful tools for genetic screening experiments. These libraries target the solute carrier family...new window) . If you have further questions or comments you can also contact them at [email protected]...

  4. CRISPR Plasmids and Resources

    Type
    Collection
    ... collections and resources are up-to-date with reagents and information you need to use CRISPR in your... for a variety of organisms, including mammals, plants, bacteria, yeast, Drosophila, zebrafish, C. elegans...of plasmids that are used together in CRISPR experiments. CRISPR Pooled Libraries : Find CRISPR libraries...knockout, activation, inhibition, and barcoding experiments. Browse CRISPR Plasmids by Function Genome Engineering.... Insert NEW CRISPR transposases insert large fragments of DNA. Transcriptional Regulation Activate dCas9...parasites. Plant CRISPR plasmids designed for use in plants. Yeast CRISPR plasmids designed for use in yeast...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson RT, Yeh J-...Addgene. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK...The number of off-target cleavage events for the VQR and VRER variants is similar to wild-type SpCas9, ...reported the development of near-PAMless Cas9 variants. These variants, named SpG and SpRY, can target NGN PAMs...the number of CRISPR reagents continues to grow, so too will the number of reagents available through Addgene...limitation: 1) the use of novel S. pyogenes Cas9 variants with varying PAM sequences, 2) the use of Cas9...positive selection screens in bacteria to identify mutants of S. pyogenes Cas9 that were able to cleave target...

  6. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...prepared in large amounts and frozen for future use or directly ligated with the annealed 24 nt oligos to generate...the spacer(20nt) on pEcgRNA plasmid as only two 24 nt oligos are required and the BsaI linearized pEcgRNA...not function properly in E. coli BL21(DE3) as transformants failed to grow after the transformation of pTargetF... This would result in the lack of recovered transformants. Our speculation motivated us to update the ...system While the above systems are great for experiments that require only single chromosomal integration...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Featured article from Journal of Visualized Experiments (JoVE) about genomic deletions in mammalian cell...video reprint from the Journal of Visualized Experiments (JoVE). The video publication by Stuart Orkin's...allows genetic perturbation of genes and genetic elements. Here we present a simple methodology for CRISPR...loss-of-function, and utility for the study of non-coding elements. This approach can be used for efficient loss-...loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol For referenced Tables...possible deletion strategies for genes and non-coding elements. For creating a gene knockout, two sgRNA located...6.3 . Assemble a 20 μl PCR with the following components: 10 μl 2x PCR mix, 0.5 μl forward primer (10 ...

  8. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...pseudovirus, where the structural and functional components of one virus (such as HIV, murine leukemia virus...information. Plasmids Expressing Spike and Spike Variants Full-length wildtype SARS-CoV-2 spike protein ...D614G change which is present in many circulating variants. The table below lists expression plasmids that...Other Resources Protocols Bloom lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Addgene COVID-19 Resources and Collections COVID...

  9. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses (LULLs)...10 business days in order to proceed with any adjustments to the original order. Turnaround Time Production...10 business days in order to proceed with any adjustments to the original order. Experimental Disclaimer...Disclaimer Due to the novel nature of experiments carried out with our materials, Addgene cannot guarantee the...assists importation by including thorough shipping documents and following import procedure, it is the responsibility...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...generates “clean” homozygous mutants with no co-integrated markers or footprints, and can be scaled up for...This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA...More details on how to use this plasmid in your experiments can be found below and in the supplemental materials... as homology arms. The system consists of two components: An all-in-one CRISPR/Cas9 vector based on the...

  11. COVID-19 Resources

    Type
    Collection
    ...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive...Libraries - Libraries of Spike (S) Ectodomain and RBD mutants (Timothy Whitehead). SARS-CoV-2 Spike Receptor ... Coronavirus Resource Hub COVID-19 SARS-CoV-2 preprints from medRxiv and bioRxiv Elsevier's Novel Coronavirus...differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome...

  12. Rett Syndrome

    Type
    Collection
    ... launched in 2008 to drive the development of treatments and cures for Rett Syndrome and related MECP2...syndrome is a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females...skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast to the sustained...there is wide variability in symptoms even within patients with the same mutation, the location and type ...2007. Correlation between clinical severity in patients with Rett syndrome with a p.R168X or p.T158M MECP2...

  13. The Pleiades Promoter Project

    Type
    Collection
    ...intron-lacZ/NLS Ple144 NTSR1 pEMS1137 EGFP/cre/NLS Ple144 NTSR1 pEMS1394 EGFP/NLS Ple146 NTSR1 pEMS1396 EGFP/NLS.../NLS Ple146 NTSR1 pEMS1617 intron-lacZ/NLS Ple147 NTSR1 pEMS1397 EGFP/NLS Ple148 OLIG1 pEMS1169 EGFP/NLS...

  14. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...systems, allowing for the integration of large fragments of DNA at specific locations. The Cas effector...recruiting the transposase (comprised of multiple components) to target locations in the genome defined by...for a ntiviral de fense), and four transposase components (TnsA, TnsB, TnsC, and TniQ). Some type I CASTs...V-K Cas effector, Cas12k, and three transposase components (TnsB, TnsC, and TniQ). Unlike type I, type V-K...

  15. TALEN Engineering

    Type
    Collection
    ...TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You may also like... Keith...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and...assembly. Links to additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN...

  16. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing...systems create a wide spectrum of single-nucleotide variants (somatic hypermutation) in a wider editing window... for expression in mammalian systems, bacteria, plants, yeast, and zebrafish. Mammalian ID Plasmid Gene...

  17. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...leaders providing research opportunities to their students and other members of the MCC community. They support...courses, at any type of institution, and at different points in their careers to participate in the community...single protein with many available isoforms and mutants provides a shared experience for the research community...PMID: 35921892 (Link opens in a new window) Acknowledgments The MCC and plasmid construction was funded...

  18. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Sarcomeric thick filaments 114414 MYL2-mEGFP AICSDP-49 mEGFP MLC-2v Sarcomeric thick filaments 124606 SOX2-...87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61 beta Endoplasmic...Troponin I, slow skeletal type Sarcomeric thin filaments 114412 TTN-mEGFP AICSDP-45 mEGFP Titin Sarcomeres...

  19. AAV Packaged on Request

    Type
    Collection
    ...mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo. It has low immunogenicity...AAV Packaged on Request, scientists can start experiments as soon as they receive their AAV prep from Addgene... processes to make sure it is ready for your experiments. Shipping 2–7 days We will email you with tracking...Once you start getting results from your AAV experiments, remember to share your protocols and results...

  20. Viral Production

    Type
    Collection
    ...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...
Showing: 61 - 80 of 1023 results