...another successful event! Addgene company culture At Addgene we organically developed the philosophy of...working at Addgene?” Most Addgenies reply with an enthusiastic, “Our company culture! Addgene is the best... Benjie Chen co-founded Addgene in 2004. They worked tirelessly to get Addgene from a concept to an up... culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the...ideas and company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time... happened naturally when Addgene was a family affair back in 2004. As Addgene has grown, it has taken ...Use it! 8. Organize If you’re doing an event that requires people to be in groups, organize your troops...

...away and here at Addgene we couldn’t be more excited! As a small, tight-knit organization we thrive on 3...this Friday will combine all of these. Addgenies have been organizing Halloween festivities for years – searching...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...

... development. These are required for a growing organism to make the complicated transition from a clump...dynamic processes are largely absent in adult organisms, where tissues structures are more stable and ...movement from the bone marrow and other lymphoid organs to specific tissues where they can scan for any...is large scale cellular movement throughout an organism. Studying cell mobility within these complex...tools to directly follow immune cells in lymphoid organs. The development of cell sorters together with ...antibodies made it possible to track cells from one organ to another. Thus several labs were able to decipher...microscopy images of chemically coloured lymphoid organs did in the past. How do researchers track fluorescently...

... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...multiple protein molecules (and also available from Addgene!) Since the signal level provided by SunTag is ...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is...pantheon of useful CRISPR/Cas9 techniques, we at Addgene are excited to see what’s coming next! References.... Find the plasmids from this publication at Addgene. Dynamic imaging of genomic loci in living human.... Find the plasmids from this publication at Addgene. A protein-tagging system for signal amplification...

...nonprofit or U.S. governmental research organizations, and organizations that require the H-1B employee to ...entity, or nonprofit research organizations or governmental research organizations. Since there is no numerical... a Good Cover Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ...Additionally, since most universities and non-profit organizations do not have the April 1 H-1B queue process or... or quota like industry, these organizations can be a good choice for individuals needing sponsorship.... in your field. Membership in a professional organization – this is not referring to one that you can ...program self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert...

...McKenna and Bert W. O’Malley. Survival of all organisms depends on efficient energy maintenance - through...nervous system signaling to peripheral effector organs, which then return information such as nutritional...processes are regulated at the cellular, tissue and organ level by nuclear receptors (NRs), their coregulators... of such signals between central and peripheral organs because of their established roles as molecular...context defined by ligand, cell type, and promoter, organizing the expression of functional groups of genes ...sections of the current NURSA website. NURSA and Addgene The great progress that has been made in the NR...literature and acquiring them in a timely manner. Addgene represents an excellent solution to the problem...

...professional works for or with an organization to evaluate the organization’s grant readiness, identify grant...stories about a time when their passion for an organization or a program and a funding opportunity coincided...submitting it, to evaluating the capacity of an organization to receive and manage funds.  Grant professionals...targeted to the grants profession? The number of organizations, both public and private, that qualify for grants...oriented nonprofits. Like Deb, I worked for an organization in a position that required some involvement...your ideal client or job will save you and the organization(s) you serve time and resources. 3. Get a mentor...Additional Resources Read other Career Posts on the Addgene Blog! ...

...them paralyzing an organization. I think these are things that I can bring to Addgene -- that comfort with...We’re happy to introduce the newest member on Addgene’s Board of Directors, Bart Newland. Newland is General...all. Q: How does your experience help you as an Addgene board member? BN: Having seen so much in the business...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...

...on the Addgene Blog Learn About Experimenting in an Open Source Lab Resources on Addgene.org Try the... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...

...post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Panel...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...

...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... produced with the pUCmini-iCAP-PHP.S plasmid (Addgene #103006) . Browse Available PHP.S AAV ID Name Promoter...

... (XFP) of different colors to label an organ or entire organism. Because each cell can have multiple copies...fluorescent proteins topic page Resources on Addgene.org Browse Addgene’s fluorescent protein collection Learn...The ability to track single cell dynamics at the organism level has been made possible by tools that allow.... Genes encoding the fluorescent proteins are organized in tandem with a single promoter, but only the...developed for tracking various cell types and organisms and for improving the level of detail possible...that allow for multicolor labeling of an entire organism or a specific tissue. XFPs are introduced using...systems allow single cell dynamics in a variety of organisms to be observed in more detail than ever before...

...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,...conference format. Additional resources on the Addgene blog Read all of our blog posts about conferences...scientific conferences have followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended...conferences (TAGC and ASGCT, respectively) and Addgenie Angela Abitua hosted a virtual Reproducibility...

...Viral Services Resources on the Addgene blog Viral Production at Addgene Viral Vectors 101: What is a Virus...Resources on Addgene.org Generating Stable Cell Lines with Lentivirus Virus Handling and Storage Addgene Viral...   Is there an easier way? In fact, there is! Addgene has a number of ready-to-use viral preps available...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific...

...Addgene Executive director, Joanne Kamens, recently participated in a Reddit AMA (short for “Ask Me Anything...but, ummmm, what is it? Reddit is a volunteer-organized platform where users can participate in forum ...trolls. 3. The AMA process was simple and well organized. You can check out the r/Science AMA Submission...only small downside to the AMA from my end as an organizer/promoter was that I didn’t have the direct link...content on many of the requested topics to the Addgene blog The blog has many other excellent posts on... science talk here). Finally, my main content organizer is the amazing app Evernote. I have a paid account...which gives me all the features I need to save, organize and easily find the resources that I come across...

...reading, and organizing Tips for writing a scientific review article   Write for the Addgene Blog ...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...method in grad school Additional resources on the Addgene blog: Tips for writing scientific manuscripts:...

...many organisms. When supplemented with adenine analogs such as 2-fluoroadenine (2-FA), organisms with ...Zurich. He is fascinated by how organisms exquisitely and robustly self-organize at the cellular and multicellular...Resources on Addgene.org Find tools for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids...Fortunately, researchers studying plants and other organisms lacking this innate immunity need not worry and...work is required, several vectors can be found at Addgene that could serve as an excellent start (#22988,...many tools for shRNA in mammals are available at Addgene. Identifying actively silenced plants Once you’...you’ve introduced your RNAi into your organism, how do you identify the actively silenced ones? Here, I’...

...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario... in bacteria, but have no fear - plasmids from Addgene depositors are making it easier than ever to use...CRISPR-induced double stranded breaks (DSB) are lethal. Addgene depositor Luciano Marraffini’s lab took advantage...CRISPR system in E. coli. The system available from Addgene consists of two plasmids: pCas9: carries Cas9 ...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted...

...plasmids with Addgene. These can be used to express genes in a variety of model organisms. Use the links...Lambda) and Higher Organisms (Cambridge, MA: Cell Press). Additional Resources on the Addgene Blog Try Out... attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for ... will depend on a number of factors, like your organism, desired expression level, and experimental purpose...expression system in just one recombination step. Addgene’s ready-made entry clones can be used with a large...of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple Lentiviral Expression... Genomic DNA and is available for all kind of organisms from mammals to E. coli. Glossary of Gateway cloning...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...discuss the importance of actionability on the Addgene blog and how SITN short-form blog posts help readers...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...Additional Resources on the Addgene Blog Resume Writing for Non-academic Science Careers 10 Steps to a Perfect...