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We narrowed to 562 results for: pac

Showing: 61 - 80 of 562 results

  1. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...receive your Packaged on Request prep 6–9 weeks after placing your order.  Can I order Packaged on Request...expanding our viral vector service to include a Packaged on Request option. Through this expansion, scientists...Figure 1: The process of requesting and ordering a Packaged on Request viral vector prep.    Excited? ...in the Addgene repository will be available for Packaged on Request service. A small subset of AAV plasmids...launch, we will offer five different serotypes for packaging: AAV1, AAV2, AAV5, AAV8, or AAV Retrograde (AAVrg...expansion happen?  You will be able to request Packaged on Request service starting in January 2025.  ...requestors.  Requesting Plasmids available for Packaged on Request consideration will have a banner on...

  2. AAV for Neuronal Tracing

    Type
    Collection
    ... Viral Vector Packaging Service AAV Monosynaptic Neuronal Tracing Viral Vector Packaging Service: Tools...17329205 Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  3. Chemogenetics AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics...Chemogenetics AAV Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  4. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated...Advantages of lentivirus include a large genetic capacity and the ability to transduce both dividing and...mutated HIV-1 provirus that couldn’t package itself due to a few missing proteins. The envelope plasmid ...terminal repeats (LTRs) that facilitate viral packaging and host genome integration. After co-transfection...the brain (Figure 1). 2nd generation lentiviral packaging system The graphic below shows how the lentiviral... infect CD4+ cells. 3rd generation lentiviral packaging system Although the 2nd generation system is safer...virus via recombination between the transfer and packaging plasmids does exist. To further reduce this possibility...

  5. Lentiviral Prep Service

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    Collection
    ...details about our viral vector packaging service, visit our Viral Vector Packaging Service page . Pooled CRISPR...lentivirus available from Addgene's viral vector packaging service. Browse constructs currently available... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR...

  6. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ..."CRISPR", short-hand for Clustered Regularly Interspaced Short Palindromic Repeats, in Pubmed returned...want to use double nickase, you could express two spacers and use PX335 to express the Cas9n (nickase). The...PAM sequence to the oligo when cloning my target spacers into the PX330 vector backbone? A2: There is no...usually adds it when they order the oligo. If your spacer sequence starts with a 'G', you naturally have ... compared to where the target site sequence (protospacer+PAM) is located? If there are other PAMs in the... there needs to be extensive homology of the protospacer followed by PAM. See Addgene's CRISPR resources... together, with each plasmid having a targeting spacer cloned into the backbone (pX330 or PX335, depending...

  7. Pipetting Protocol

    Type
    Protocol
    ...to the amount you are dispensing can negatively impact your experimental results. This protocol will guide...

  8. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...cardboard packaging, there are some less obvious improvements that reduce the environmental impact. Using...waste from packaging Made from 100% post-consumer recycled material Reduced cardboard packaging Plant-based..., all kit components and packaging Yes, most kit components and packaging Yes, most kit components...to increase your output. But, exceeding a kit’s capacity can actually reduce your ultimate plasmid yield...ensure proper lysis, but you will still face column capacity limitations since the columns can be “clogged”...magnet as you scale up. Table 1: Standard Prep Kit Capacities* Prep size Amount of culture Approximate ... are looking to reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable...

  9. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ...with alternating spacers and repeats, was the original impetus for studying CRISPR. Spacers: Copies of foreign...sequences are called protospacers. Repeats: Bacterial DNA that appears between each spacer in the CRISPR array...the CRISPR array and include a spacer and a repeat sequence. The spacer sequence is the RNA guide, like...real mouthful of a phrase: Clustered Regularly Interspaced Short Palindromic Repeats. That contrast of simplicity...composed of identical repeat sequences and variable spacer sequences. The array is transcribed and processed... into crRNAs, each including one repeat and one spacer. In bacteria, these crRNAs are bound by Cas proteins... sequence base pairs with the tracrRNA, and the spacer sequence is used to target complementary DNA sequences...

  10. Viral Vectors

    Type
    Collection
    ...host genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses...type, linked above. Virus Expression Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell...

  11. Control AAV Preps

    Type
    Collection
    ... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control AAV Browse... Page . Don’t See What You’re Looking For? Our Packaged on Request service offers you even more options...Please note this does not guarantee viral vector packaging service, but lets us know what viruses would be...

  12. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...well, though. Viral packaging and entry The genetic cargo requires viral packaging to infiltrate and integrate...from the transfer plasmid. Viral packaging and entry The viral packaging proteins for AAV are rep and cap...Addgene blog Parts of the AAV packaging plasmid Overview of Lentiviral Packaging Plasmids Adenoviral production...multiple plasmids with many components are used to package lab grown viruses. It can be intimidating at first...transfer plasmid while the other plasmids with packaging or replication components are called helper plasmids...). These LTRs define the limit of what will be packaged. The 5’ LTR and 3’ LTR, respectively, act as RNA...nucleocapsid viral components needed for viral packaging, assembly, and display. Pol, the last component...

  13. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... Viral Vector Packaging Service Penn Vector Core Transfer Viral Vector Packaging Service: Penn Vector...Optogenetics Scott Sternson AV-1-20071P 20071-AAV1 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-1-...pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden AV-9-20071P 20071-AAV9 pACAGW-ChR2-Venus-AAV Optogenetics Karel Svoboda AV-9-...-tdtomato Scott Sternson AV-5-20071P 20071-AAV5 pACAGW-ChR2-Venus-AAV Karel Svoboda AV-5-20949P 20949-...

  14. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...have different packaging capacities depending on the virus. AAV has a packaging capacity of ~4.7 kb, while...containing your gene of interest plus two packaging plasmids (three packaging plasmids for 3rd generation lentivirus...Baculovirus transfer plasmids have a much larger packaging capacity — up to 38 kb! Generally, insect cell production...lentivirus production). The packaging plasmids are widely available, so you’ll only have to worry about cloning...while lentivirus has a capacity of 8–10 kb. Time Viral production in mammalian cells is relatively quick...construct, called a bacmid. Like AAV and lentiviral packaging plasmids, bacmids are readily available, so the...if needed # of plasmids Three or four Two Packaging limit ~4.7 kb (AAV); 8–10 kb (lenti) 38 kb Cells...

  15. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...into an AAV vector is challenging due to its packaging capacity of AAV (~4.7 kb). While this approach has...previously packaged Cas9 and multiple gRNAs into separate AAV vectors, increasing overall packaging capacity...preferential infection of certain tissues. However, packaging Streptococcus pyogenes (SpCas9) and a gRNA together... the only CRISPR enzyme that’s small enough to package into AAV. At 984 amino acids in length, Cas9 from...Cas9 (NmeCas9) at 1,082 amino acids, can also be packaged into AAV. NmeCas9 has the added advantage that...respectively, the size of both is within AAV’s packaging limit. For CRISPR-based RNA editing, the REPAIR...approximately 4.1 kb in length, allowing them to be packaged in AAV.   Split AAV approaches  While finding...

  18. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...high capacity AdV) are devoid of all viral coding sequences and retain only the ITRs and packaging signal... Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are devoid of viral genes and therefore...kb. Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly...such as HEK293 cells. 1st generation AdV cloning capacity is limited to 8.2 kb, and in vivo transgene expression...RCA) can be generated if the E1 gene from the packaging cell line is transferred into the AdV by recombination...Gen AdVs were designed to have increased cloning capacity, decreased  potential for the generation of RCA...2nd generation AdVs, thereby increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...contaminating your transduction with the packaging cells. Packaging cell debris can be removed either by ... particles in a smaller volume. Always remove packaging cell debris before proceeding to ultracentrifugation...step (5 minutes at 300-500 g). After removing packaging cell debris, virus can be pelleted by ultracentrifugation...reduce the volume of cell culture medium on the packaging cells immediately following transfection. In other...fold (Stockschläder et al., 1999). 5. Check your packaging cells for infection If your transductions aren... virus is being generated by your transfected packaging cells. This check can be performed routinely and...disrupting the flow of your experiment. If your packaging cells have been successfully transfected and produced...

  20. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...Service picks up mail. Recycle plastic packaging Packaging is a huge contributor to the increase in ...lot of their plastic packaging (from Corning®, Falcon®, or Axygen® product packaging) for free! It has to...the lab could make a big difference in our global impact as scientists. Many scientists are already leading...toward responsibility in our lab’s environmental impact, but we can all do more to make research more environmentally...your address sticker off the box, remove the ice pack (it’s not recyclable), tape the box shut, and drop... to be from their company and it has to be packaging (no coated tissue culture plates, for example). Check...and companies have the potential to make a huge impact directly on waste, but also on culture. Encourage...
Showing: 61 - 80 of 562 results