Prime Editing: Adding Precision and Flexibility to CRISPR Editing
Type
Blog Post
...without double strand breaks (DSBs) or donor DNA templates. How does this work? First, an engineered prime...reverse transcriptase domain uses the pegRNA to template reverse transcription of the desired edit, directly...increase activity, enhance binding between the template and primer binding site, increase processivity...different genomic sites compared to PE1.
The pegRNA: A template and guide all in one
The other important component...pegRNA is a guide RNA that also encodes the RT template, which includes the desired edit and homology ...remake that strand using the edited strand as the template.
A third prime editing system called PE3 does ...increase thermostability through tighter binding to template-primer." Nucleic acids research 37.2 (2008): 473...