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We narrowed to 303 results for: rigi

Showing: 61 - 80 of 303 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ... approach, and conclusions (see above). Keep original files in a separate folder: You will often use...for a single use, save the script as originalName_descriptionOfEdit in the same directory where it was ...that was temporarily used. Just be sure that the original code remains in tact! Take notes Comment in ...

  2. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...per cell division and thus, genes closer to the origin of replication will be overrepresented compared...divided. This means that genes located near the origin will be over-represented compared to those further...promoters drove maximal gene expression near genomic origins of replication, the stabilized promoters created...

  5. Of Myc and Men

    Type
    Blog Post
    ...Do you ever wonder about the origins of some of the common techniques or tools you use in the lab? Take...we explore… the life and times of the myc tag.  Origins of protein tags For about as long as the disciplines...mouse IgE, goat IgG, (and more!) as well as the original mouse IgG1. And the refinement of this trusty ...

  6. MXS Chaining

    Type
    Blog Post
    ... cell migration (Sladitschek and Neveu, 2015). Origin and purpose of MXS chaining MXS-chaining was designed...digestion followed by ligation. Upon ligation, the original pattern of the MCS is regenerated at the flanks...approaches in mammalian cell culture systems (their original goal). Their work has demonstrated that the MXS-chaining...

  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...indicates that the cancer has spread from where it originated to another organ in the body. Such misdiagnosis... cells that may have spread beyond the point of origin, and radiation to stop or slow the growth of tumors... before it starts to spread beyond its point of origin. One of the most exciting developments recently...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...relating to reproducing the original research can be documented -- in the original context in which it was...import Word documents into RSpace, maintaining the original formatting. In this way data is moved from a static...

  9. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...machinery making an original C-G pair converted to T-A or reverted to the original C-G by base excision... ‘G’ by cellular polymerases. Subsequently, an original genomic A-T base pair is converted to a G-C pair...

  10. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to solve your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford...starting point, their ideas scored 37% higher in originality. It may not be easy to optimize virus production...References and Resources References Grant, A. (2017). Originals how non-conformists move the world. Penguin Publishing...

  11. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...tools and assumptions to reveal findings that the originator was oblivious to, no matter how much time they...productivity”, so this is unlikely. Usually, the originating scientist and colleagues are privileged in viewing...generators. This practice doesn’t appear to harm the originating researchers, even if they are occasionally beaten...

  12. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Originally written by Marcy Patrick and Mary Gearing on Mar 12, 2015; updated by Christina Mork, Jul ...DNA template used for repair is identical to the original DNA sequence at the DSB, or it can introduce specific...newly synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...through equal opportunities for all regardless of origin. It is therefore essential that internationalization...experience from talented professionals to their original countries to help build, together with the universities...frontiers. Moreover, sharing a culture/country of origin with an international mentor creates a closer and...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... really need to use FACS (like they did in the original publication). I thought I could see GFP expression...against GFP expression, but I didn’t. 2. In the original publication they see GFP expression in about 20%... cells at an MOI of 0.3, as recommended by the original publication. Pro-Tip: 1. Use multiple gRNAs to...

  15. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ... started engineering a CBE to be worse at its original job. They removed the uracil glycosylase inhibitor...could explain why the edited U base opposite the original G is not primarily converted back to a complementary... to improve chromatin accessibility One of the original labs that pioneered these editors (calling them...

  18. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...the process a bit easier and more efficient. Our original policy was straightforward, but cumbersome: depositors...champagne or cupcakes, or maybe the lab member originally responsible for the deposit had already moved...

  19. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...known to be genetically manipulated, its anatomical origin, if cell line has ever been the target of an ‘-...about. If you have cell lines without a certified origin (you know, that split you got from the graduate...

  20. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...The resulting chimeric antibody would have the original variable regions from the parental rat antibody...antibody retains the binding specificity to the original target and can be used with secondary antibodies...
Showing: 61 - 80 of 303 results