Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 323 results for: sars

Showing: 61 - 80 of 323 results

  1. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...a deep understanding of retroviral biology is necessary in order to use the vectors correctly. A solid.... The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation ... G. M., & Temin, H. M. (1974). Infectious rous sarcoma virus and reticuloendotheliosis virus DNAs. Journal...M. (1973). RNA-directed DNA polymerase of Rous sarcoma virus: Initiation of synthesis with 70 S viral ...

  2. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...the minigenome contains all cis-acting signals necessary to direct minigenome replication and reporter ...vectors) because they do not encode all of the necessary viral proteins. Minigenome systems have been developed...standpoint, a plasmid with all four genes and necessary control elements for each would be relatively ...transfected into cells. All of these components are necessary to efficiently reconstitute a functional viral...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...Illumina MiSeq sequencer enabled us to perform the necessary sequencing on-site, but now we were presented ...meant that we already had a clear idea of the necessary steps to perform:      Fig. 2: Quick-stepping...internal software infrastructure; however, it is not necessary to use containers with Nextflow. For each of the... in a cloud environment. Given the throughput necessary to keep up with our sequencing instrument (hundreds...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...pause during elongation which may actually be necessary for proper protein folding (Stadler and Fire, ...synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always...Santana-Quintero LV, Katneni U, Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource for codon usage.../doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution of synonymous...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...voltage indicators: QuasAr1 and QuasAr2 (quality superior to Arch). Both QuasAr1 and QuasAr2 possess mutations...DNA-guided optogenetic tools; however, targeting the necessary light-activated protein domains to the appropriate...kinetics. By combining both the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch...Arch indicator (pJMK004), as well as the CheRiff, QuasAr, and Optopatch vectors available to the scientific...

  6. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, & Zafira Castano ...and all over the world, she thought that it was necessary to develop a single platform to connect talented...students experienced great improvements, not necessarily quantifiable, but always significant. Doubts,... her on twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at...

  8. Kit Free RNA Extraction

    Type
    Protocol
    ...Sodium Citrate, pH 7.0 0.5% (wt/vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar...

  9. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...truthfully and in clear terms. It isn’t necessary to be cruel, but is necessary to be direct and clear. There is... too long, a small correction can become an unnecessarily big issue for you and for the employee. Make...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...increase the utility of CRISPR/Cas9, it will be necessary to improve its multiplexing capacity. Multiplexing..., the modification of multiple genes is often necessary. Guide RNAs (gRNAs) are commonly packaged in 400...bacteria), and only a few elements of tRNAs are necessary for recognition and cleavage. This sequence flexibility...

  11. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...BioRender template. Since DnaA binding is necessary to initiate replication, controlling the amount...for the host, as no additional components are necessary. Iterons are not the only tool to stringently ...differ in the specialized proteins or sequences necessary for initiation or the mechanisms that regulate...

  12. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...training behind you (and largely only that is necessary), there are opportunities to work for biotechnology...their technical support, and a PhD is not always necessary. The job description and the candidate requirements...empathetic, and tactful. Occasionally it can also be necessary to take the lead and be assertive. You develop...

  14. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...what constitutes the “appropriate” codon is not necessarily obvious. You might think, “Nonsense! I should... above, not every region of a protein should necessarily be translated rapidly to produce a protein that...optimize a gene with one of these tools, it doesn’t necessarily mean the gene is going to express well. If you...

  15. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...eBook Here 1. Have a competitive CV You don't necessarily need a Nature/Cell/Science paper. You do need...can suffocate an exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed...an interview, your previous success will not necessarily translate into getting a job. Your papers and...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plant-specific changes to CRISPR components are necessary to enable genome editing in plant cells.   This...modifications to commonly used CRISPR plasmids are necessary to use the CRISPR system in plant cells. Like ...Ti "helper" plasmid, which contains Vir genes necessary for T-DNA processing and insertion into the plant...

  17. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...abundance, and thus difficult to purify in quantities necessary for study. Moreover, the ability to isolate purified...strain for pSer-incorporation The second component necessary for phosphoserine incorporation is a modified ...interest as possible, some optimization will be necessary. To begin, one should optimize protein production...

  18. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...enough funds to get started, wasting funds on unnecessary materials, or even a loss of the offer all together...usually focus on the numbers, rather than the necessary resources. This is why at Experimental Designs...too often, we see early investigators making unnecessary short term sacrifices that hurt their goals, ...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...and validation Can’t forget the controls! The necessary control experiments will vary depending on your...standard IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  ...Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour, S., Varma, A., Walavalkar, K., Wissink, E. M...

  20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...reproducibility. For a study to be “reproducible” does not necessarily mean that someone has replicated it precisely...them upon completing an experiment. There’s not necessarily in-principle acceptance, but readers of pre-registered...practices including talking to statisticians when necessary and encouraging interlab peer review of experimental...
Showing: 61 - 80 of 323 results