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We narrowed to 95 results for: t aav

Showing: 61 - 80 of 95 results

  1. CRISPR Guide

    Type
    Collection
    ...INTEGRATE ( I nsertion of T ransposable E lements by G uide R NA- A ssisted T argeting), developed by Samuel...Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M...PMID: 28041849 Sakuma, T., Nishikawa, A., Kume, S., Chayama, K., & Yamamoto, T. (2014). Multiplex genome... R. T., Silverstein, R. A., Amrani, N., Peng, C., Kramme, C., Savic, N., Pacesa, M., Rodríguez, T. C.,... (22), 5635-5652.e29. PMID: 34653350 Chu, V. T., Weber, T., Wefers, B., Wurst, W., Sander, S., Rajewsky... Gao, L., Cox, D. B. T., Yan, W. X., Manteiga, J. C., Schneider, M. W., Yamano, T., Nishimasu, H., Nureki...Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3 induces...

  2. Mammalian RNAi Tools

    Type
    Collection
    ... a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression...window) . Moore, C. B., Guthrie, E. H., Huang, M. T., Taxman, D. J. (2010). Short hairpin RNA (shRNA):...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 ...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID: 27052166 Nishimura...containing ten guides against each of the 8,158 predicted T. gondii protein-coding genes). Dr. Lourido’s studies...are conserved among apicomplexans and important for T. gondii fitness. Sixteen of these genes were investigated...was shown to be important for the initiation of the T. gondii infection, and necessary for the asexual cycle...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...effects on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...10.1016/j.neuron.2016.01.040 Takeda S, Kadowaki S, Haga T, Takaesu H, Mitaku S (2002) Identification of G protein-coupled...

  6. Retrovirus Plasmids

    Type
    Collection
    ...γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook γ-Retrovirus (gamma-retrovirus...1780 pBABE-neo largeTcDNA MoMLV Expression of Large T antigen for creation of immortalized cells (see Weinberg...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...your experimental values and your controls. ANOVA, t-test, multiple regression analysis, and non-parametric...Hellemans J; Huggett J; Kubista M; Mueller R; Nolan T; Pfaffl MW; Shipley GL; Vandesompele J; Wittwer CT... https://doi.org/10.1038/s41598-018-26707-8Suzuki T; Higgins PJ; Crawford DR. Control selection for RNA...Thelwell N; Millington S; Solinas A; Booth J; Brown T. Mode of action and application of Scorpion primers...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9.../doi.org/10.1002/0471142905.hg1508s88 Leidy-Davis T, Cheng K, Goodwin LO, Morgan JL, Juan WC, Roca X, ...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin...

  10. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems....2017.07.037 Li C, Psatha N, Gil S, Wang H, Papayannopoulou T, Lieber A (2018) HDAd5/35++ Adenovirus Vector Expressing...

  11. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Resources on the Addgene blog   Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview .... S., Mokhtar, U., Machado, L. R., Abdul Aziz, A. T., Shueb, R. H., Zaid, M., Sim, B., Mustafa, M., Nik...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase.../10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer T, Wu A, Mouravlev A, Carpenter JC, Snowball A, Knauss...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ...

  13. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias...

  14. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...QCascade’s genomic target site.  T-RL= transposon right-left orientation. T-LR= transposons left-right orientation... advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, ...

  15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...respectively, to obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The...Anzalone, A. V., Gao, X. D., Podracky, C. J., Nelson, A. T., Koblan, L. W., Raguram, A., Levy, J. M., Mercer,...McCreary, J., Pierce, S. E., Tolar, J., Meissner, T. B., Chaikof, E. L., Osborn, M. J., & Liu, D. R. (...://doi.org/10.1038/s41586-024-07259-6 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, ...

  16. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...Thy1-YFP-H mice).  However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...Pubmed. A. Ertürk, C.P. Mauch, F. Hellal, F. Förstner, T. Keck,  K. Becker, et al. (2011). Nature Medicine,...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter...

  17. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-mTagRFP-T AICSDP-22 mTagRFP-T Centrin-2...PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1... Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP-69 mTagRFP-T Nucleophosmin Nucleolus (granular component...114403 LMNB1-mTagRFP-T AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36...References Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, Nelson A, Hookway C, Ludmann...mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of ...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in AAV Dual promoter Separate...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate...expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page Lentiviral Can transduce both dividing...Ubiquitin pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter...

  19. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on... Chuhma N, Mingote S, Yetnikoff L, Kalmbach A, Ma T, Ztaou S, Sienna AC, Tepler S, Poulin JF, Ansorge ...Müller K, Fenno L, Kim YS, Ramakrishnan C, Andrási T, Deisseroth K, Holmes A, Hájos N. 2019. Excitation... C, Dorrier CE, Tipton GJ, Ramakrishnan C, Kozicz T, Deisseroth K, Thiele TE, McElligott ZA, Holmes A,... Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None...None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None...Items 55641 pAAV-Ef1a-fDIO EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and to compare different Cas enzymes in all-in-one AAV delivery. While Fragmid developers were specifically...Fragmid toolkit to facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo...Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024...
Showing: 61 - 80 of 95 results