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We narrowed to 955 results for: ARC;

Showing: 391 - 400 of 955 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...including over 3,500 human TF isoforms, to help researchers understand this regulatory network. The transcription...library of each unique TF open reading frame (ORF), barcoded for tractability. These ORFs are individually ...generated (Joung et al., 2023). Fig. 1: Barcoded TF library was packaged in lentiviral particles... and screening mechanisms, although the unique barcodes for each TF help streamline identification. While... test prophylactics on and a program that any researcher could use to generate the relevant disease model...

  2. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...experiments and other researchers by implementing reproducibility best practices in your research. For a place ...GHP_HarvardChan — Shekhar Saxena (@ssaxenageneva) March 16, 2020 Give back to your community. For those...experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future experiments...contribute data to the AAV Data Hub to help the research community. Set expectations for your productivity...

  3. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...meaning, making it difficult or impossible for researchers to track down that resource. A more long-term...information, and more. The plasmid page also helps researchers find other publications that have used the same...support standardized persistent identification of research reagents, several journals or organizations now...several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs) which are supported...repository are verified and identifiable to save researchers time and make their work more reproducible. While...

  4. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...was contributed by Marco Straccia, an Associate Professor at University of Barcelona. The challenge: Making...fre-sci.info Marco Straccia is currently an Associate Professor at University of Barcelona. He is also ..., I and other professors at the University of Barcelona wanted to develop strategies to make our classes...

  5. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...Loren Looger’s lab at HHMI Janelia Research Campus to give researchers the ability to monitor glutamate ...iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following: Improve sensor’s photophysical.... In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3 in two additional anchoring...Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only...

  6. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    ...guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 (Cas9) is an...strategies to manipulate that process. We began our research with a simple question: how do Cas9 and sgRNA ...donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating DNA repair ...delivery of purified Cas9 ribonucleoproteins." Genome research 24.6 (2014): 1012-1019. PubMed PMID: 24696461....Our CRISPR Guide Find CRISPR Plasmids for Your Research Check out Our List of gRNA Design Tools ...

  7. Components of CRISPR/Cas9

    Type
    Blog Post
    ...requirements. These different PAM requirements allow researchers to target many different genomic loci. 3. Cleave.... When using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct...elements separated by unique sequences. When researchers first discovered these arrays, they did not know...them “spacers” (see figure below). After years of research, we now know that each direct repeat, combined...leaving a F) double-strand break. A “gRNA” is a researcher-designed hybrid of the tracrRNA and the crRNA...

  8. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...story of base transversion editors starts with researchers in the Keith Joung lab, who were engineering ...editing between bacteria and mammalian cells Researchers in the Xueli Zhang lab were making similar observations...develop more efficient and precise editors. Most researchers agreed on the first part of the likely editing... efficacy varied among target sequences. The researchers hypothesized that the CGBE edit process occurs...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify....1038/nbt.4172 Kurt, I. C., Zhou, R., Iyer, S., Garcia, S. P., Miller, B. R., Langner, L. M., Grünewald...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...continue to accelerate research and discovery by improving access to useful research materials and information...have been lost to continue to be available to the research community.    Validation For most cloning projects...construct by Sanger sequence analysis. But for this research, it was likely that the sequence of the functional...range secondary antibodies are commonly used in research laboratories to detect the different subclasses...

  10. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...iPS cells and embryos make a case for conducting research in embryos should our society decide that germline...not being used to establish a pregnancy, the researchers were able to examine each individual blastomere...embryos and iPS models, it appears that embryo research is the only way to answer questions of efficiency...Given moral and ethical concerns surrounding such research, it is crucial that scientists proceed with caution...non-biologists alike. Given the public significance of this research, we have the obligation to discuss it as openly...
Showing: 391 - 400 of 955 results