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We narrowed to 1,046 results for: FER

Showing: 781 - 800 of 1046 results

  1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    .... Note: Jennifer Tsang and Mary Gearing contributed to the update of this post. References Abudayyeh...2016). Type VI CRISPR systems, which target RNA, offer additional targeting flexibility. Cas13a (formerly...

  2. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...0-5%. This blog post covers a brief review of different basic BE technologies and their adaptation for...Nickase Cas9 (D10A) Deaminase PAM requirements Reference CBE SpCas9 rAPOBEC1 NGG 6 SpCas9 Target-AID...improvement in the laboratory of Yinong Yang at PSU.   References 1. Komor, Alexis C., et al. "Programmable editing...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...tracking experiments (Rizzo, 2009). With many different FPs that emit across the visible spectrum, researchers...antibodies. The “spaghetti monster” name is a reference to the deity of the social movement, the church... to the expanded use of smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance...

  4. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...these discoveries, scientists have studied how different parts of MECP2 influence Rett Syndrome. Zoghbi...varying degrees determine the clinical severity of different MECP2 mutations. More recently, the Bird lab found..., Rett databases, and other helpful tools.  References: Amir RE, Van den Veyver IB, Wan M, Tran CQ, ...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...below, certain specialized strains are available to confer greater transcriptional control, assist with proper...pLys comes in two flavors—pLysS and pLysE—the difference being that the latter provides tighter control...minimal media supplemented with M9 salts may be preferable if the protein product is secreted to the medium...

  6. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...host organism. Because transcription machinery differs between cell types or organisms, promoters must...eukaryotes and prokaryotes We have included two reference tables below listing some of the most common bacterial...Mammalian Vectors Read about Reporter Gene like Luciferase and GFP Resources on Addgene.org Find Plasmids...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...dice on a new gRNA in the future! References and Resources References Eva K. Brinkman, Tao Chen, Mario ... frame editing frequency is 50%, then you could infer that approximately 25% of cells are likely -/-, ...

  8. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...recent research @daria_gift_shch.     References and resources References Shemetov, A.A., Oliinyk, O.S. & ...the monomeric state of a probe leads to less interference with the oligomeric state of a fusion partner...

  9. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...Cas12a, Cas13a does not require strict sequence preferences at the target site, while Cas12a requires a PAM...femto-aptomolar range. For more details on the differences between these methods, check out this table.  ...can follow her on Twitter @shra_krishna.       References Azhar, M., Phutela, R., Ansari, A. H., Sinha,...

  10. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...leaves a “scar” in the chromosome, sometimes interfering with expression of surrounding genes. However...Gram-negative bacteria become resistant to antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa...for allelic exchange Read our molecular biology reference Watch molecular biology protocol videos ...

  11. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...binding sites increased activation of HIV promoter-luciferase constructs. In multiple HIV-1 latent T cell lines...the HIV-1 copy number, although the efficiency differed from 48-100% between strains. In a similar experiment...for some of the world’s most common diseases. References Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski...

  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...on unnecessary materials, or even a loss of the offer all together. The problem is that the discussions...specialized equipment, try finding institutes that offer shared equipment that you can use at a lower cost...turned down a faculty position because none of the offering institutes were able to provide the resources ...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...control of one of seven enhancers specific for different interneuron populations. The enhancers span all...(LAMP5) — as well as cholinergic (choline acetyltransferase, ChAT) neurons. The authors characterized ...effort to develop molecular tools for studying different brain cell types. Look for more of these interneuron-targeting...

  15. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...irrespective of in silico prediction. These techniques differ in important ways, but can also complement one ...sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer to the clinic...https://magazine.scienceforthepeople.org/.     References 1. Fu, Yanfang, et al. "High-frequency off-target...

  17. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...Twitter feed will be all about CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start... Jennifer Tsang...

  18. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ... this new technology as helpful as we have!  References and Resources Additional resources on the Addgene...Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ...viral vector information. Each day, we’ll cover a different topic as shown in the graphic below. Follow the... Jennifer Tsang...
Showing: 781 - 800 of 1046 results