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Addgene
Showing: 781 - 800 of 1008 results

  1. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...throughout an organism’s genome. These reporters have historically been used extensively in fly, mouse, and yeast.... These are effective tools but neglect some biologically relevant situations. Tissue-specific genes...

  2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene The cap gene... of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248–265 . https://doi.org/10.1016...

  3. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...blogosphere is to read and comment on their blogs. 5)   Local science museums are a great place to find opportunities...Science Communication Program at the University of California, Santa Cruz, has been turning scientists into...

  4. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...

  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...

  8. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    ...distribution. I don’t have space in this blog to call out every Addgene Team because they’re all doing...requests for scientists doing almost every type of biomedical science research. Our work accelerates and enhances...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...of lox sites which are part of a dual lox system called FLEX or DIO. Lox sites are recognized by the recombinase...

  10. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...Randolph-Habecker J (2013). A Flexible Mouse-On-Mouse Immunohistochemical Staining Technique Adaptable to Biotin-Free...Tuson JR, Pascoe EW, Jacob DA (1990) A novel immunohistochemical technique for demonstration of specific binding...

  11. Plasmid Cloning by PCR

    Type
    Blog Post
    ...analyzing the gene’s function. The process is shown graphically in the following figure, in which we add EcoRI...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...type; however, their usage has been limited by technical problems. For instance, it’s much more difficult...presence of the CPP may alter protein function or localization. Thus, the usefulness of CPPs must be evaluated...

  14. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...mentoring meetings a priority. Schedule meetings or calls regularly and don’t cancel, so that you drive progress...

  16. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...aqueous samples might be affected by dry ice, and specifically how dry ice can affect virus from Addgene. ...never stored on dry ice/exposed to CO2 (Fig. 5). Basically, the CO2 goes in and the CO2 goes out. Conclusions...

  17. Components of CRISPR/Cas9

    Type
    Blog Post
    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...arrays, they did not know their functions and simply called the repeated elements “direct repeats” and the ...

  18. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability... and “Sequence” tabs. BLAST Use the Basic Local Alignment Search Tool (BLAST) via the NCBI website...

  19. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...
Showing: 781 - 800 of 1008 results