We narrowed to 1,002 results for: Des

... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

...with (purple) or without (green) ligands they are designed to detect. Photo courtesy of Kristina DeYong, ...

...community and support a wide range of experimental designs. Here are nine great additions, including our first...

...Plasmids 101 blog series Learn about the different grades of DNA  Read about the various ways to quantify...

...pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics Adesnik New viral vector pAAV-nEF-Con/Foff DREADD Gi-...

...Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi and Ben describe their projects 0:28 - 1:12 : Introduction from...

... which results in neutralization or targeted destruction.  Antibodies are composed of two light chains...

...Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench...

...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...

...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...

...that the most cited work in history is a 1951 description of an assay to determine the amount of protein...

...oligo overlap cloning, you can design a set of oligos containing your desired restriction sites and add them...that will pay off for years to come. Design Briefly, we will design overlapping oligos that once annealed...procedure will simply differ in terms of primer design). Let's assume that your favorite vector has a ...digest of existing sites in the original vector. Designing oligos To add NdeI, PacI, AscI and MfeI sites ...between the EcoRI and SalI sites of the vector, we design a top oligo with each of the additional sites in...complementary C of the other oligo), but this would destroy the EcoRI and SalI sites in the final vector. Order...

...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

...Introductory techniques designed to help you get started in the lab. Name Description (Link opens in a new...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...blocks for many more complicated procedures. Name Description (Link opens in a new window) Link to Video Making...constructing and analyzing your plasmids. Name Description (Link opens in a new window) Link to Video Restriction...titering and testing your virus preparations. Name Description Link to Video General Transfection Introduce ...Protocols for common antibody applications. Name Description Link to Video Transfection Introduce plasmid ...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

...,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. The...

...our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral preps! Download...

...job of providing skills training for scientists destined to continue in academia. It seems it’s largely...

...work with any group of trainees interested in designing career exploration programs or peer mentoring ...

...are usually unresolved and small, so I suggest designing your primer at least 50bp upstream of the sequence...