We narrowed to 998 results for: Des

...Breakdown (Time : Topic) 0:00 - 0:28 : Niroshi and Ben describe their projects 0:28 - 1:12 : Introduction from...

... which results in neutralization or targeted destruction.  Antibodies are composed of two light chains...

...Addgene's Most Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench...

...travels from 2015, including a great European photo slideshow from our UK team member Benoit Giquel. Bluegene...

...information in our lab software, labeling stabs with barcodes as they go. Stabs are organized by shipment and...

...that the most cited work in history is a 1951 description of an assay to determine the amount of protein...

...Gel Procedure Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link...sequence. FAQ How do I design primers? See our reference page on how to design primers for detailed instructions...Description of Polymerase Chain Reaction with protocol, tips and FAQ Protocols...DNA and the Taq polymerase, PCR requires free nucleotides [dNTPs; adenine (A), cytosine (C), guanine (G...the denaturing. Your 5’ and 3’ primers should be designed to have similar melting temperatures (Tm). Set...protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes. What do I do if my... to work efficiently. Taq Buffer with MgCl 2 : Provides an optimal and stable chemical environment for...

... 1: Buffer exchange using a desalting column The 10 mL Zeba Spin Desalting Columns can accommodate up ...This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using...Western Blot Protocol Introduction This protocol describes how to affinity purify recombinant antibodies ...L2884 Aprotinin, Millipore Sigma A6279 Zeba Spin Desalting Columns, 7 kDa MWCO, 10 mL, Thermo Fisher 89893... buffer exchange using a Zeba Spin 7 kDa MWCO desalting column. If the concentration of the pooled sample...into 2 columns. Twist off the 10 mL Zeba Spin Desalting Column’s bottom closure and loosen cap. Place ...Spectrophotometer to see if the sample has reached the desired concentration. If the sample concentration is still...

...our virus backgrounds. Addgene’s viral service provides ready-to-use AAV and lentiviral preps! Download...

...other uses.   Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

...,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. The...

...job of providing skills training for scientists destined to continue in academia. It seems it’s largely...

...work with any group of trainees interested in designing career exploration programs or peer mentoring ...

...are usually unresolved and small, so I suggest designing your primer at least 50bp upstream of the sequence...

...press release. "In the future, it could allow us to design versions of these editing tools that are more specific...

...University Center for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing...

...think is the most important resource that Addgene provides the scientific community? Access to plasmids which...

...databases, DNA sequence manipulation, and primer design. Best wishes for the new year and your research...

... who started the BALSA group, a nonprofit that provides short-term consulting services. Initiative and...

...University Technology Managers (AUTM). This MTA provides many benefits for both the providing and receiving...