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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,022 results for: NTS

Showing: 781 - 800 of 1022 results

  1. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...exception to this effect -- meticulously designed experiments in labs around the world were put on hold indefinitely...the likelihood in our Poisson process into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential...corresponding factor 𝜙z if t is during event z. The "events" we considered for our model were weekends, Christmas...

  3. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...ccdB gene die off and a higher percentage of transformants contain the ccdB-less construct. Other toxin-antitoxin...described, the hok/sok systems consist of three components: Host killing (hok), a long-living toxin that...selective pressure is necessary or for long-term experiments. They’ve also helped researchers design and optimize...

  4. How to Write a Scientific Review Article

    Type
    Blog Post
    ...remember where you read particular studies or experiments. Later, you can easily convert the citation style...much you can get done between office hours and experiments. A comment about citing: I wasn’t sure if I should... be revolutionary, but you could include a few comments on where you think the field is going or what ...

  5. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...because ‘antibiotic’ merely means that the drug prevents the growth of a living organism. As for multi-...making them more difficult to treat in the future. Patients not finishing the whole course of antibiotics ... can utilize tactics like rotating antibiotic treatments, so that exposure to each antibiotic is minimized...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...rounded out the top four average video abstract view counts.” For researchers looking to garner more attention...can be shared by research scientists, faculty, students, private sector researchers, or pretty much anyone...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...

  7. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...silencing required addition of chromatic-opening agents to boost expression to appreciable levels. We wanted...expression and robustness without chromatin-opening agents. It ended up being very straightforward to engineer...for improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via ...

  8. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...what does this mean for a molecular biologist who wants to express genes from one organism in another? Let...organism that contains expression limiting regulatory elements or use a non-canonical code (Gustafsson et al....optimization should not be used in some in vivo experiments and in biotechnology and therapeutics (Mauro ...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    ...community obtained high-quality data for our DNA-based reagents. Not only do we extract as much information as...system that we chose needed to fulfill certain requirements: Customizable to our specific needs Modern ...-well plate basis, but for rapid testing and refinements, we specified a select small number of input ...

  10. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    ...copy number of the plasmid increases, increased amounts of these antisense RNAs inhibit plasmid replication...The concentration of negative regulation system elements in the cell is artificially high for both plasmids... uncommon, or perhaps a synthetic biologist who wants to develop a plasmid-based tool that is easy to ...

  11. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...sequential homologous recombination events, but because these events are rare, each requires selection....efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization...

  12. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs...parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized plasmid....template). Due to the small difference in size, the fragments should be separated on a high percentage agarose...

  13. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    ..., ligases (notably T4 DNA ligase) are critical reagents for many molecular biology protocols, including...substrates containing no gaps, and will not join any fragments end-to-end without the exo/polymerase generation...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...

  14. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...the march for science on April 22, 2017. While experiments (and editors) can move slowly, news about possible...set up with various science demonstrations, and students could walk around and ask scientists questions... how planets form around young stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com...

  15. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization Once generating...of reprogramming, and 2) studies with clinical endpoints. In the first scenario, robust and efficient generation...Let us know about your iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru ...

  17. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...can save researchers time by quickly relaying key points about an unfamiliar topic or lab process. Along...you don’t see here? Give us suggestions in the comments section below! Additional Resources on the Addgene...

  18. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...conversations with PIs, lab managers, and grad students. We’ve talked about their hot-off-the-presses ...Investigator. How-to guides for troubleshooting your experiments and getting better results at the bench, with...

  20. Our New Antibody Mascot is....

    Type
    Blog Post
    ...as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been following a set of clues ...yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend We need your help...
Showing: 781 - 800 of 1022 results