We narrowed to 1,015 results for: NTS

...silencing required addition of chromatic-opening agents to boost expression to appreciable levels. We wanted...expression and robustness without chromatin-opening agents. It ended up being very straightforward to engineer...for improved purity and contrast. For in vivo experiments, simply induce injected engineered cells via ...

...remember where you read particular studies or experiments. Later, you can easily convert the citation style...much you can get done between office hours and experiments. A comment about citing: I wasn’t sure if I should... be revolutionary, but you could include a few comments on where you think the field is going or what ...

...sequential homologous recombination events, but because these events are rare, each requires selection....efficient method to generate precise, unmarked mutants, but it frequently requires considerable optimization...

...remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs...parental plasmid provides a mean for selecting for transformants which have taken up the mutagenized plasmid....template). Due to the small difference in size, the fragments should be separated on a high percentage agarose...

..., ligases (notably T4 DNA ligase) are critical reagents for many molecular biology protocols, including...substrates containing no gaps, and will not join any fragments end-to-end without the exo/polymerase generation...and the ATP dependent 9°NTM DNA Ligase are not replacements for T4 DNA Ligase in applications such as routine...

...the march for science on April 22, 2017. While experiments (and editors) can move slowly, news about possible...set up with various science demonstrations, and students could walk around and ask scientists questions... how planets form around young stars and the instruments we use to study them. You can follow her at alexandrazgreenbaum.wordpress.com...

...reprogramming proteins needed to perform these experiments. Assays for iPSCs Characterization Once generating...of reprogramming, and 2) studies with clinical endpoints. In the first scenario, robust and efficient generation...Let us know about your iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru ...

...decreased immunological markers of stress in adolescents. It also put those young people in a better mood...list? Let us know by sharing it with us in the comments.   ...

...can save researchers time by quickly relaying key points about an unfamiliar topic or lab process. Along...you don’t see here? Give us suggestions in the comments section below! Additional Resources on the Addgene...

...conversations with PIs, lab managers, and grad students. We’ve talked about their hot-off-the-presses ...Investigator. How-to guides for troubleshooting your experiments and getting better results at the bench, with...

...as-yet–unnamed friend is a huge fan of scavenger hunts. In fact, they’ve been following a set of clues ...yet: Addgene will soon be distributing affinity reagents. Name Our New Antibody Friend We need your help...

...how they were created, and how to use them in experiments. Best practices!). Making the find plasmids ...even easier and more useful, let us know in the comments. I hope this helps! Additional Resources on the...

...MicrobiGals created glow in the dark petri dish ornaments and microbe cookies! 🤗 🤗 🤗 We hope everyone...the hashtag to see the description of all the ornaments. #DeckTheLab @Addgene pic.twitter.com/GsAlD6sjM6...

...without spending time and resources in preparative experiments.  We’re excited to begin offering this service...of Tris buffer suitable for molecular biology experiments such as cloning, digestions, PCR etc. and/or ...

... vector services allow scientists to conduct experiments faster, skipping the preparation steps of production... serotype, and prep size they need for their experiments — and we’ve been able to ship them out in record...

...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...

...four of the key figures Number of requests per reagents (as a horizontal bar chart) Number of requests...requestors, year of first reagent sent, year with most reagents sent, and the most recent full calendar year ...

... the most widely used class of protein-binding reagents. In the lab, their binding specificity allows ...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips that allow...

...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These...

...level with the addition of two light-sensitive ingredients - CRY2 and CIB1 – which they borrowed from Arabidopsis...explained. “It helps us adapt to influences in our environments. We are able to react and learn. All of this...