Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,082 results for: ica;

Showing: 781 - 800 of 1082 results

  1. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...favorite twitter #agarart accounts, Petri dish Picasso (@PetriPicasso) came together and delivered very impressive...are some of our 2017 contest winners:   Chicagoland's Community Biology Lab (@ChiTownBio) and one ...Mackin Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a case of holiday cheer...

  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...simple in C. elegans and bacteria. But CRISPRing typical mouse models, including creating Cre-dependent ...on both sides and the T7 promoter on one end. Typically, Easi-CRISPR works best with insertions of 2 kb...models varies from 8.5-100% of pups born, with typical efficiencies of 30-60%. Quadros et al. attribute...2.5x more animals than i-GONAD and is a more technically difficult process. Overall, the main drawback... out our CRISPR Guide Find Plasmids for Cas9 Purification Find CRISPR Plasmids for Mammalian Expression...

  3. Call for Guest Bloggers

    Type
    Blog Post
    ...any molecular biologist Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career...source of information on recent advances in the biological sciences and techniques that simplify and expedite... help hone your blog post for our audience of biological scientists from a variety of different fields...

  4. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    ...Addgene podcast, we sit down with Alex Chavez, a Clinical Fellow in Pathology at Masachusetts General Hospital...Church and Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD...and explains why he loved the first 2 years of medical school. 7:05 - 10:55 : Alex's focus during graduate...postdoc 12:01 - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to...

  5. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland. After completing...completing her PhD in Chemistry and Chemical Engineering with Harry Gray at Caltech, Crystal moved on to a ...Chapter 7: How Crystal developed her science communication skills and how they've helped her at Novartis...scientific teams at the Novartis Institutes for BioMedical Research to develop novel biologics for the treatment...

  6. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    ...acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase, EcoRI...scientists to continue to do excellent work in the biological sciences. Future awardees Now that this first...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

  7. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...infectious disease once limited to small pockets in Africa has spread to urban areas and across the ocean....percent of the most recent cases, primarily in West African countries. Even that grim estimate might prove ...protein present on the virion surface – making it critical for viral attachment and fusion. Once inside a...glycoprotein’s role in infection also makes it a critical component of vaccines and the target of neutralizing...

  8. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...analyzing your cells using flow cytometry, you are typically measuring the presence or absence of certain markers...reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will only occur...free amines on and inside the cells and are thus typically used for distinguishing live from dead cells. ...describe a basic panel (Table 2, Figure 5) for a hypothetical experiment in the lab. Let's say I am culturing...Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating negative...

  9. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the user. This approach is useful for several applications as will be discussed below. The software could...has a maximum of one gene knocked out. In this genetically heterogeneous population, cells behaving differently... you could majorly accelerate progression into clinical trials. Secondary screens  If you have generated...genes you’d like to test for phenotypic effects. Typically the phenotype would be studied in vitro, but there...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs...

  10. Reflections and Looking Ahead

    Type
    Blog Post
    ...Antibody characterization is critical to enhance reproducibility in biomedical research. eLife, 13, e100211...tell only part of the story. This year, we made significant strides in making science more accessible and...launched AAV Packaged on Request, a service that dramatically expands and simplifies your viral vector options...

  11. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...promoter. It can be used to drive Cre expression specifically in neurons. AAV Delivered Cre - A useful tool...Indeed, this AAV provides you with a means to specifically activate expression of any Cre-dependent gene...pmSyn1-EBFP-Cre Of course there are also more complicated ways to use this particular AAV. For instance...Additionally, you could use the retrograde form to specifically express Cre in neurons with axons that extend...

  12. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...service. A small subset of AAV plasmids will have technical or legal restrictions that make them ineligible...be reviewed by Addgene for legal approvals and technical compatibility with our production process. This...request is approved, you will receive an email notification that your request is ready to order. An “Add...found in our in-stock preps. If a request has technical considerations that may affect expected titers...

  13. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...incredibly useful molecular biology tool, but like any biological system, it has certain drawbacks. First, the ...Gerald Rubin’s lab sought to make complex genome modifications in Drosophila using multiple recombinases. To...offspring carried both the recombinase and GAL4, indicating that the recombinases were inherited normally...Chinese hamster ovary (CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin...

  14. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    ...  “This enables you to do customized genetic modification on a scale that was really not possible before...genome engineering with CRISPRs was limited to modification of a few loci at a time,” Wang said. “It was... lentiviral libraries and their demonstrated application offer proof-of-principle that CRISPR-Cas9 can...aimed for 300 cells per construct, but in some biological systems one “might need larger numbers.” Further...

  15. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...: Find sequences for plasmids assembled from publications and commercially available sources, as well ... as expression patterns, post-translational modifications and polymorphisms Codon optimization tool: To...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer...

  16. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...time for me to complete an online social media certification on social media data, and I was given a fantastic... occasional TikTok. I was also responsible for typical entry level tasks such as stuffing new depositor...connections with coworkers seem, in retrospect, nonsensical. I have found the people at Addgene have become... at Northeastern and Addgene co-op studying communications, marketing, and global fashion studies. She...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...service organization like the Red Cross or the American Cancer Society, or maybe you think about a local...services we provide like quality control, curation, technical service and open-access educational resources....decisions at Addgene If you’re ever curious what specifically drives the decision making process at Addgene...Gizela, Henry, Ina, Inna, Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly...

  18. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...symposia on Sunday afternoon were dedicated to science communication through art. Addgene Advisory Board...page and, just a few weeks ago, we attended the American Society for Microbiology's annual meeting (ASM... Jessica Welch...

  19. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ... perspective – a plasmid-containing cell must replicate the plasmid in addition to its own chromosomal...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...cloning. In these instances, the cloning vector typically has two separate resistance cassettes and your...and can be used in place of Ampicillin in most applications. Antibiotics vary in their sensitivitly to heat...

  20. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ... lab at ETH Zürich. “Instead of a graphical abstract, it’s a musical abstract,” says Di Ventura, a professor...in @nchembio Title: Bye bye L-arabinose drive. Dedicated to Edo, Armin and @KhammashLab. I hope you all...reached the level of expression needed for most applications.   Figure 1: With blue light, the VVD...
Showing: 781 - 800 of 1082 results