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We narrowed to 1,046 results for: sta

Showing: 781 - 800 of 1046 results

  1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid could be ...cells gave drastic improvement in expression. Stabilizing the gvpA transcript with WPRE reduced the number...days. GVs should be visible under phase contrast starting day 2 after transfection.     Fig: 2: Expressing...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...of its parts. Elements such as the antibiotic resistance gene and the origin of replication are necessary...means exhaustive, but should be a good place to start when trying to pick your perfect promoter! Eukaryotic... gene Although this list is a great place to start, the tables above do not delve into the tissue or...

  4. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system works in ...needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. For further temporal...19396156. PubMed Central PMCID: PMC3655711. 4. Gronostajski, R M, and P D Sadowski. 1985. “The FLP Recombinase...

  5. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...efficiency is at least in part due to La binding and stabilizing the 3’ end of the pegRNA, which is not well protected...pegRNAs (epegRNAs), which add a structural motif to stabilize the 3’ end of the pegRNA. As research has just... research; however, microglia are notoriously resistant to viral transduction. Through in vivo screening...

  6. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...NGS-based amplicon sequencing. To use the tool, you start by uploading your Sanger sequencing files, either...useful measure for those who are interested in understanding how many of the contributing indels are likely...CRISPR analysis tool is part of Synthego’s full stack Genome Engineering Solutions, which also includes...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...screening experiments as well). Therefore, if you’ve started down the CRISPR path, you’ve probably got a gene...insert this region into a pCAG-EGXXFP plasmid using standard cloning techniques (The XX indicates where your... them in mouse embryos. When researchers first started using CRISPR/Cas9 for genome editing in mice, they...

  8. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...study is an excellent example in which the authors started with a brain region that was known to be generally...steep learning curve and many opportunities for mistakes. The easiest way to learn optogenetics is straight...shared with all. Due to the versatility and standardization of genetic tools in the laboratory mouse, most...

  10. Evolution of Lab Techniques

    Type
    Blog Post
    ...amplification process, rather than just at the end as in standard PCR. This allows the researcher to compare different... interest to create a physical map of the DNA. Starting in the 1970s, restriction mapping was commonly...techniques we use now may soon become obsolete. For instance, the popular genome-engineering tool, CRISPR, ...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...Sharp PA, Zhang F. In vivo genome editing using Staphylococcus aureus Cas9. Nature. 2015 Apr 9;520(7546);186...

  12. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...appropriate inducing agent (0.4 mM IPTG is a good start if you’re using a pET vector) and antibiotic for...protein purification, but overnight at 30°C is a good start. Prepare a lysis solution containing your favourite... solution (around 108 CFU/mL is a good place to start) and add your membranes to the solution, shaking...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...transversion editor The story of base transversion editors starts with researchers in the Keith Joung lab, who were...as AP sites. To explore this further, the team started engineering a CBE to be worse at its original ...before the field begins to converge on a set of standard tools. Additionally, aspiring genetic editors ...

  15. Addgene is Stopping Use of X (formerly Twitter)

    Type
    Blog Post
    ...Bluesky: @addgene.bsky.social; or @addgene on Instagram, TikTok, Facebook, and LinkedIn. We are also exploring...and features that previously made the platform a mainstay of conversation in the scientific community. Addgene...

  16. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ...Deck The Lab contest submissions on Twitter and Instagram and we’re excited to share these creative entries...frog is coming to town. @Addgene @UMich_MCDB #christmastaco #frogsnowflake #xenopus #ASCBEMBO19 #MCDB 🎄...

  17. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...Addgene! And we can’t think of any better way to start off our holiday season than by thanking the twelve...Priyamvada Prathima, who wrote a helpful guide to understanding your flow data. Flow Cytometry Readouts Toj...

  18. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Breeding, Genetics, and Biotechnology at Michigan State University with the backing of a prestigious Fulbright... : Kingdom's biotechnology research at Michigan State University 9:36-10:29 : Kingdom's transition back...

  19. Summer Fun at Addgene!

    Type
    Blog Post
    ...Social Media Are you following us on Twitter or Instagram (or Facebook or LinkedIn) but want to hear and...love sunshine and warm weather - check out our Instagram all summer long to see what kind of shenanigans...

  20. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...annual tradition in which we choose our teams and start brainstorming months in advance. Our teams comb ...into an outfit?), and of course there’s always nostalgia (old board games and movies can provide great ...
Showing: 781 - 800 of 1046 results