...and teaching community through the TRAINED initiative (Translating Research on Arabidopsis Into a Network...teachers’ preference for using animal systems to demonstrate biological concepts - and you have yourself a...experiments that include growing plants require pots, trays, soil, fertilizer, seeds and a growth space with...

...brain science, my curiosity did not ultimately translate to a desire to do the research itself. Several...enjoyed a taste of what was to offer, continued straight on to a master's degree in science communication...venturing forth from tertiary study, the myriad trajectories ahead can be condensed into two discernible ...stroll from my office to the tearoom would often illustrate the breadth of knowledge and passion on display...

... available materials is growing along with a centralized information resource for scientists studying ...studying the coronavirus. Addgene’s Technology Transfer Team also moved fast to ensure that the majority of deposited...could keep up with demand and keep new projects on track to succeed at the same time. I began approaching...needs of scientists worldwide with Addgene’s traditional excellence through whatever lies ahead this year...

...experimental details (e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid...helpful because viewers will know exactly which strain you used so if they wish to replicate your experiment...experiment, they know exactly where to get that strain. Experimental design & methods: These questions are... Using AAV Technologies Using AAV for neuronal tracing Resources on Addgene.org Read our AAV guide Browse...

...ordering viral vectors. Virus production can be a frustrating process that requires a large investment of time...have experience with it. We’re reducing this frustration and getting researchers to the experiments they...applications where we have delivered them in vivo to the central nervous system. This service will further enable... titer lentiviral preps, troubleshoot viral transductions, and overviews for beginners just starting work...

... the value of having a mentor, developed some strategies for finding mentors and, perhaps, asked someone...can take is to set goals to work on together and track progress at every meeting. It can take many mentoring...Development Plan or mentoring journal will help a Mentee track progress, adjust expectations, and record milestones...to complete action items and make progress. To illustrate this point, consider that I try to exercise every...

...They found that aptamer insertion in the gRNA tetraloop (a four base hairpin loop motif within the gRNA...inserted an octet of MS2 hairpins into the gRNA tetraloop and went through subsequent design iterations ...10 (2015): 3002-7. PubMed PMID: 25713381 PubMed Central PMCID: PMC4364232. Ma, Hanhui et al. “Multiplexed... (2016): 528-530. PubMed PMID: 27088723 PubMed Central PMCID: 4864854. Ma, Hanhui et al. “CRISPR-Sirius...

...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The Nuffield Council...stages of human clinical trials. This model also demonstrates that when we are at the point of scientifically...question we are left with is not “what is the translational pathway for CRISPR/Cas9 research” but whether...

... Have you ever found yourself frustrated reading through a paper to find that there is insufficient information... mistakes in this can lead to wasted time or retractions. Using a catalog number for identifying a reagent...making it difficult or impossible for researchers to track down that resource. A more long-term solution is...IDs in published work allows readers to quickly track down the specific plasmids used. RRIDs and resolvable...

...thicker peptidoglycan layer, gram positive bacteria trap the crystal violet stain within their membrane, ... added, it dehydrates the peptidoglycan layer, trapping the large dye complex created by the interaction...retain the stain, leading to its decolorization. In contrast, counterstain, safranin, is a light and water ...Blue-white screeningPlasmids 101: Common lab E. Coli strains  ...

...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...Jennifer Doudna. “They are the first direct demonstration of use of genome editing to correct point mutations...patients with cystic fibrosis. They were able to demonstrate functional correction of the gene in clonally...Potential of CRISPR/Cas9 Gene Editing as a Treatment Strategy for Alzheimer’s Disease.” Journal of Alzheimer's...

... been quite the administrative headache receiving constant email requests and tracking their fulfillment... ones that are druggable) is a key step in our strategy to throw a therapeutic monkeywrench into the virus... sites - so cloning projects should be fun and straight-forward. All constructs are tagged with a 2xStrep-tag...

...these experiments because developing embryos are transparent, so it’s easy to watch development in real time...NIR light.  NIR wavelengths have better tissue penetration than shorter wavelengths, which makes it possible...10.1038/nmeth.3735. PubMed PMID: 26808669. PubMed Central PMCID: PMC4916159. 2. Jacobson, K., Rajfur, Z.,...j.tcb.2008.07.001. PubMed PMID: 18706812. PubMed Central PMCID: PMC4445427. 3. Bulina, M. E., Chudakov, ...

... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore...MC, Fitchmun MI. Protein instability following transport or storage on dry ice. Nat Methods. 2013 Apr;10... Aug 13;1:14034. PubMed PMID: 26015974. PubMed Central PMCID: PMC4420252 Additional Resources on the Addgene...

...of prominent members of the bioscience and tech transfer communities who help guide and promote Addgene...overall mission of making science easier and more transparent. That’s largely my job [at the Broad] too… to...role in the future, Doench says, Addgene is “a central resource for what is worth having. The work that...critical function really…. Addgene’s role as a central repository for what is useful and what was used...

...from a dcm or dam methylation-deficient bacterial strain such as JM110 or INV110. Avoid star activity. Some...DNA and allows you to visualize the DNA under ultraviolet (UV) light. Don’t forget to add loading buffer... estimate how far down the gel your bands have traveled based on the dye! Always run a ladder. Ladders...of larger DNA fragments. I hope these tips demonstrate that plasmid verification is not just necessary...

...Washington DC (#SfN17). This massive conference attracted over 30,000 attendees (scientists and nonscientists...sampling of the broad variety of tools in neuroscience trade. There were, of course, many companies selling antibodies...people ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around...experiments and to find tools that will make your transition into the field much easier! If there’s a plasmid...

...research more visibility.    Figure 1: Illustration of SARS-CoV-2 from Philippa Steinberg for the...glycoproteins and other proteins that require post-translational modification may not be easily expressed in ...sequencing as a first pass QC. The lab team manually transforms, innoculates, and performs DNA minipreps for ...order to bring them inline with our slower, more traditional, QC process. While this has worked well for the...

... host cells (the simplest example would be a transformed mesophilic, for example E. coli, carrying a plasmid...higher levels of growth compared to the E. coli strain expressing the empty vector. And thus was born ...working towards widening the applicability of our strategy using the pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a...blogger Sreepadmanabh M from IISER Bhopal. All illustrations / figures featured were designed by our team...

...26343581. PubMed Central PMCID: PMC4597191. 5. Eyquem, Justin, et al. "Targeting a CAR to the TRAC locus with...the Memorial Sloan Kettering Cancer Center. In contrast to using randomly integrating viral vectors, CRISPR...(2009): 110-116. PubMed PMID: 19122659. PubMed Central PMCID: PMC2671237. Additional Resources on the ...