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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 801 - 807 of 807 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...decades. Wildtype gamma-retroviruses have been engineered into gamma-retroviral vectors that can be safely...safely used in laboratory settings. These engineered gamma-retroviral vectors have many aspects that make...lines (Figure 3B). These cell lines have been engineered to stably express gag-pol and/or env , eliminating...deletion prevents further transcription of the full-length virus. The potential for oncogenesis is largely...Bosch, V., Nocken, F., Merget-Millitzer, H., Engelstädter, M., Kurth, R., Groner, B., & Cichutek, K. (...deliver genetic material into different host cells. Engineered for safety and efficiency. Content last reviewed...

  2. Molecular Biology Reference

    Type
    Guide
    ...and other genetic elements using specifically engineered plasmids, commonly referred to as vectors, which...promoter (e.g., a liver-specific promoter). The strength of the promoter is also important for controlling...that can drive expression of short RNAs. Genome Engineering Plasmids - Used to target and edit genomes. Genome...Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. coli B F dcm ompT hsdS(rB mB) gal ccdB...traDelta36 proAB lacIqZDeltaM15] NEB Stable New England Biolabs F' proA+B+ lacIq ∆(lacZ)M15 zzf::Tn10 (...group of newly synthesized DNA strands of varying lengths whose last nucleotide is labeled. Once all the ...

  3. Plan Your Experiment

    Type
    Guide
    ...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...them. To increase specificity, consider using an engineered Cas enzyme with higher fidelity. In addition ...gRNAs that have been used successfully in genome engineering experiments. Using validated gRNAs can save your...References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system. Methods in Molecular...Glass, Z., Lee, M., Li, Y., & Xu, Q. (2018). Engineering the delivery system for CRISPR-Based genome editing...

  4. Science Guides

    Type
    Guide
    ...Chemogenetics is a technique that uses genetically engineered receptors to selectively control and investigate...system in bacteria, have been modified for genome engineering. Find essential background information on CRISPR...Optogenetics Optogenetics integrates optics and genetic engineering to measure and manipulate cells (frequently neurons...

  5. Cloning

    Type
    Guide
    ...Cloning Resources Empty Backbone Guide Genome Engineering Guide Restriction Enzyme Gateway TOPO Gibson ... its multiple cloning site . Due to their short length, it is also easy to add restriction sites to any...custom ordered DNA oligos of 60-80 base pairs in length, with 30-40 base pairs of homology to the ends ...

  6. Educational Resources

    Type
    Guide
    ...molecular biology techniques. Topics include: Genome Engineering Fluorescent Proteins CRISPR Viral Vectors Plasmid...

  7. Promoters

    Type
    Guide
    ...into a protein. RNA polymerase II transcribes messenger RNA (mRNA) which is the RNA responsible for providing...
Showing: 801 - 807 of 807 results