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  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...The health of the HEK293T cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the...

  6. Retrovirus Guide

    Type
    Guide
    ...envelope, Phoenix-AMPHO (for the infection of mammalian cells.) Using this system, virus is produced in... host cell cytoplasm, the viral componentes are small enough to enter the nucleus through nuclear pore...

  7. Lentiviral Guide

    Type
    Guide
    ...express cDNA from a lentiviral transfer plasmids normally used for shRNA expression? Yes, it is feasible...viral particle, it is not possible for viruses normally produced from these systems to replicate and produce...

  8. Optogenetics Guide

    Type
    Guide
    ... viral vector with a floxed opsin in a VGAT-cre animal will result in expression of the opsin only in ...optically assembled protein architectures in living mammalian cells. LITE Two-hybrid system integrating the ...

  9. Science Guides

    Type
    Guide
    ...pathways. Receptors are remotely controlled via small molecules, which allows for specific control of ...

  10. Adenovirus Guide

    Type
    Guide
    ...Are adenovirus and AAV different? Yes! AAV is a small single-stranded DNA parvovirus and was discovered...

  11. Sequencing Primers

    Type
    Guide
    ...GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) 3' end of maltose binding protein, forward primer mCherry-F CCCCGTAATGCAGAAGAAGA...
Showing: 801 - 811 of 811 results