We narrowed to 818 results for: Mal

1. Sequencing Primers

   Type

   Guide

   ...GATGAAGCCCTGAAAGACGCGCAG (Waugh lab) 3' end of maltose binding protein, forward primer mCherry-F CCCCGTAATGCAGAAGAAGA...

2. Pouring LB Agar Plates

   Type

   Protocol

   ...~220 mL) just in case we spill anything or have small errors in measurement. You should also always make...-mix slightly. Even so, you should always use thermally insulated gloves when removing anything from the...

3. Protocol - How to Design Primers

   Type

   Protocol

   ... Short primers are mainly used for amplifying a small, simple fragment of DNA. On the other hand, a long...

4. Protocol - How to Create a Bacterial Glycerol Stock

   Type

   Protocol

   ...plasmid DNA prep the following day. Tips and FAQ The optimal concentration of long-term glycerol storage is ...

5. Protocol - How to Streak a Plate

   Type

   Protocol

   ...toothpicks or wire loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar ...

6. Protocol - How to Purify DNA from an Agarose Gel

   Type

   Protocol

   ...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

7. Protocol - How to Perform a Diagnostic Digest

   Type

   Protocol

   ...-8 pieces such that all (or most) fragments are small enough to be accurately sized on a gel and also ...

8. Centrifugation

   Type

   Protocol

   ...height. They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...

9. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ... hour. Using this ratio, you can calculate the minimal amount of enzyme for your reaction. However, keep...

10. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

    ...for most standard cloning (where the insert is smaller than the vector) a 3 insert : 1 vector molar ratio...

11. Lentivirus Production

    Type

    Protocol

    ...discard the tube and thaw a new working stock. The optimal mass DNA:mass PEI ratio will need to be empirically...

12. Transfection for Recombinant Antibodies

    Type

    Protocol

    ...the tube and vortex for 5 s to mix. Pro-Tip The optimal ratio of DNA:PEI may vary significantly and should...

13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type

    Protocol

    ...Importantly, iodixanol is inert and non-toxic to mammalian cells. Therefore, and unlike other density gradient...

14. Protocol - How to Run an Agarose Gel

    Type

    Protocol

    ...higher percentage agarose gel will help resolve smaller bands from each other, and a lower percentage gel...

15. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...be placed into a backbone vector of choice with minimal limitations. Background In its simplest form, PCR...

16. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type

    Protocol

    ...mix. Centrifuge at 3100 x g for 8 min . Remove a small aliquot of your sample from the filter reservoir...

17. Antibody Validation Using the Indirect ELISA Method

    Type

    Protocol

    ...incubation and plate read Tips and Troubleshooting The optimal concentration of primary antibody to use will vary...

18. AAV Production in HEK293 Cells

    Type

    Protocol

    ...The health of the HEK293T cells is critical for optimal AAV yield. Do not overgrow your cells. Pass the...