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We narrowed to 861 results for: SOM

Showing: 801 - 820 of 861 results

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Plasmids 101: Modular Cloning

Type

Blog Post

...which a precursor molecule is made, modified (sometimes many times), and isolated afterwards. To engineer...
Progress Towards a PAM-Free CRISPR

Type

Blog Post

...looser or different requirements. Luckily, there are some available. Cas proteins that don’t utilize NGG...
Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Type

Blog Post

...Workshop 2016 At past symposia, FoR worked to highlight some of the problems facing postdocs and graduate students...
Cpf1 Update: Comparison to Cas9 and NgAgo

Type

Blog Post

...discovered, researchers wondered if it could overcome some of Cas9’s shortcomings. Cpf1’s preferred PAM is ...
Plasmids 101: FLEx Vectors

Type

Blog Post

...allowing for rescued expression of GeneR. Sometimes also referred to as DiO (Double-floxed inverse...
Tips for Titering Your Lentiviral Preps

Type

Blog Post

...comparing different batches of virus. In addition, some viral preps are very sensitive to freeze thaw cycles...
AAV Vector Quality Control: Going the Extra Mile with NGS

Type

Blog Post

...make mistakes. What if a tube is mislabeled or someone grabs the wrong plasmid? What if bacteria snuck...
Truncated gRNAs for Regulating Gene Expression

Type

Blog Post

...truncated gRNAs (≤15nt) have been found to retain some ability to induce Cas9 to make edits (AC, WLC, and...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...of the targeted gene will be removed from the chromosome and and replaced by the resistance gene.The gene...
xCas9: Engineering a CRISPR Variant with PAM Flexibility

Type

Blog Post

...actually lower for xCas9 3.6/3.7 than for SpCas9. For some non-promiscuous gRNAs, xCas9 3.7 displayed more ...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Type

Blog Post

...blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find other CRISPR...
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

Type

Blog Post

...substrates, it demonstrates significant activity on some mismatch-containing pairings. During Golden Gate...
PCR Applications for Copy Number Variation Assays

Type

Blog Post

...experiment? Complexity of the gene of interest Some gene sequences are harder to amplify and quantify...
Hot Plasmids and Viral Preps - May 2021

Type

Blog Post

...collection to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Axon...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...discovery of many cellular mechanisms, there are some limitations to working with FPs. Overexpression ...
Genome-wide Screening Using CRISPR

Type

Blog Post

...used to perform genome-wide screens and highlight some of the reagents that have been made publicly available...
Light Sheet Fluorescence Microscopy

Type

Blog Post

...of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...
Tips for Using BLAST to Verify Plasmids

Type

Blog Post

...reaction is often unreliable, we suggest using the “Somewhat similar sequences (blastn)” program for blastn...
CRISPR 101: Ribonucleoprotein (RNP) Delivery

Type

Blog Post

... be incredibly valuable to scientists, there are some potential complications that must be considered ...
Isolating Genomic Regions of Interest with the CRISPR System

Type

Blog Post

...numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition...

Showing: 801 - 820 of 861 results

Results per page:

Plasmids 101: Modular Cloning

Progress Towards a PAM-Free CRISPR

Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

Cpf1 Update: Comparison to Cas9 and NgAgo

Plasmids 101: FLEx Vectors

Tips for Titering Your Lentiviral Preps

AAV Vector Quality Control: Going the Extra Mile with NGS

Truncated gRNAs for Regulating Gene Expression

Plasmids 101: Knockout/Knock-In Plasmids

xCas9: Engineering a CRISPR Variant with PAM Flexibility

CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

PCR Applications for Copy Number Variation Assays

Hot Plasmids and Viral Preps - May 2021

Fluorescent Tagging of Endogenous Genes with SapTrap

Genome-wide Screening Using CRISPR

Light Sheet Fluorescence Microscopy

Tips for Using BLAST to Verify Plasmids

CRISPR 101: Ribonucleoprotein (RNP) Delivery

Isolating Genomic Regions of Interest with the CRISPR System

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