... can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight. ...DNA will vary upon intended use. Typically yields range from several hundred micrograms to a milligram of...taken to perform adequate NGS based QC to ensure no change in representation compared to the pre-amplified...

...material that you are weighing by looking for a weight range on the scale. Make sure that the weight of the material... material you’re weighing out is within this range. If you need less than a gram of material, use an analytical...

...that will help to maintain the plasmid without rearrangement of the plasmid DNA. Additionally, specific treatments...competent cells. Pro-Tips Commercial competent cells range significantly in their transformation efficiency...

... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL of the diluted antibody... antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL fluorescently-labeled...

...the VSV-G envelope provides the widest tropism or range of cells a virus can infect. Overview of Retroviral...and γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, ... vice versa. Envelope plasmids may be used interchangeably. For more information about lentivirus, please...

...prokaryotic promoters. Eukaryotic promoters span a wide range of DNA sequences. It is not unusual to have several...an inducer molecule binds to the repressor, it changes its conformation, preventing its binding to the...our popular Plasmids 101 series , covers topics ranging from the newest breakthroughs in plasmid technologies...

...much as several miligrams and at concentrations ranging from 150 ng/μL to several μg/μL. The protocol below...pellet (can be done by inverting the tube at an angle over kimwipe) for 20-30 min. Resuspend pellet with...

...reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template...polymerase that can withstand radical temperature changes during a typical PCR. The DNA polymerase has an...

... Edit Generate a specific user-defined sequence change in a particular gene, such as generating a point...was successful. PCR amplification, subcloning and Sanger sequencing (for HDR or NHEJ): Provides semi-quantitative...Validating your Genome Edit . Publications Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9...

.... Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

...protocols, our educational resources cover a wide range of topics in molecular biology, laboratory research...Protocols Find written and video protocols for a wide range of applications in molecular biology, plasmid cloning...

...Browse the article from Ran et al. , 2015 from the Zhang Lab to find plasmids optimized for use in AAV, or...it displays very high infectivity across a broad range of cell types. The mutant AAV-DJ8 displays the properties...

...measured under the 260/280 column (A good purity ranges from 1.80-2.00). Repeat for each sample. Notes:...

... streak #1. Pro-Tips Hold your tooth pick at an angle, the way you would hold a pencil, so that you can...

...note we have found that there is generally a broad range of antibiotic concentrations that will work for ...

... gels, so you will want to stay in the 0.7-0.8% range if possible. You will want nice crisp bands. This...

... bacterial endonucleases that recognize a large range of DNA sequences. Given the variety of these enzymes...

...practical lab protocols that you can use for a wide range of applications, with videos for select protocols...

...Data Figure 1: 293T cells were transduced with a range of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h ...

...contaminated with pathogens or chemicals that present a danger to people and the environment in biohazard containers...