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  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...of large-scale assembly useful, you may ask? You might be surprised by how multi-purpose it can be. Let... Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with NEB’s DAD Interview...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...are a multitude of known AAV serotypes that have slight variations in their viral capsids. Novel forms ...AAV types, AAV1, 2, 3 and 5 are thought to be as high as 70% among the human population. These pre-existing...

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...1948 as a variation of the more commonly observed light emission by fluorescence. The widespread use of ...distances that are too miniscule for conventional light microscopy. Don't FRET - Use these FRET resources...

  4. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...for these RNA sequences (3 nM), however, was quite high and above what would be expected in patient samples...daunting. It took a true team effort and many late nights, not unlike most research, but the urgent need ...

  5. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...fold (Davis et al., 2002). However, polybrene is highly sensitive to freeze-thaw cycles and should be stored...resistance marker expression following transfection, higher-level expression after approximately 72 hours is...

  6. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...expression can change as well. The more frequently and tightly bound DNA is to histones, the more compacted it...occurs at CpG islands (regions of the DNA with a high frequency of C and G nucleotides). The conversion...

  8. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...my experience the best set-up is panelists in a slight semi-circle (so they can see each other) on comfortable...redirect the conversation. The person you cut off might be miffed but the other 200 audience members will...

  11. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...think I need. Unlike lentivirus, AAV requires a higher number of viral particles for efficient gene transfer...only harvest the cell pellet. My yield would be higher if I used PEG precipitation, but for my experiments...

  14. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Gamma-retrovirus genome (left) and an HIV lentiviral genome (right.)  HIV seems an unlikely candidate for use as...genomes in one retroviral particle: pseudodiploidy and high rate of genetic recombination. Proceedings of the...

  15. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...wanted to think more closely about the wealth of high-throughput sequencing (HTS) data we generate and...the aim of the journal club is to begin building a high level understanding across multiple teams or to ...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...bp, but pay attention to the sequence’s Tm, as a higher Tm give more efficient recombination. We normally... two colonies for simple cloning procedures. Use high fidelity DNA polymerase This will minimize random...

  17. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...cumbersome to use and lead to lower viral titers due to higher plasmid number. For more information on the key...Lentiviral particles with the VSV-G envelope have high infectivity in a wide range of cell types, so they...

  18. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...professionals identify grant opportunities with the highest likelihood of funding and develop a strategy and... project management, and program evaluation are highly transferable. Use your experience and your passion...
Showing: 801 - 820 of 980 results