We narrowed to 847 results for: tat

1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   ... The team found that "all-in-one" Nanoblades facilitated HDR with either a ssDNA or dsDNA donor template...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

3. 5 Tips for Troubleshooting Viral Transductions

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   ... to a target cell by reducing the repulsive electrostatic forces between these two negatively-charged ...

4. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...sophisticated genome-wide analysis. At many of its computationally predicted off-target sites, Cpf1 does not mediate...

5. Plasmids 101: Terminators and PolyA signals

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   ...sequence conserved Rho-independent termination annotation for 90 bacterial elements. By Ppgardne at English...

6. How-to: 5 Steps to a Great Panel Discussion

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   ...use any slides for the event. Slides are for presentations, not discussions. I will rarely work with a ...

7. Hot Plasmids: Winter 2025

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   ... and subthreshold events, including miniature excitatory postsynaptic potentials (mEPSPs) (Hao and Lee...

8. Common Injection Routes in Mice

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   ...finally, to expert.   Figure 1. Visual representation of common injection routes in mice. Image created...

9. Production of Virus in Insect Versus Mammalian Cells

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   ...eukaryotic organisms. Typically, what system is used is dictated by the specific type of virus you are trying to...

10. Viral Vectors 101: Voltage Indicators

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    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

11. Transferable Skills: Negotiation

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    ...more important to you and why? What are their expectations/needs and how are they different from mine? ...

12. AAV Vector Quality Control: Going the Extra Mile with NGS

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    ...is Addgene plasmid #44362. Method validation, limitations, and future plans How did AAV-68544 become contaminated...

13. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...Associated Plasmids from Subach et al. Protein Excitation (nm) Emission (nm) Brightness pKa Maturation ...

14. 10 Basic tips for mammalian cell culture

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    ...their comfort zone around 37 °C and 5% CO2 and get agitated with sudden changes in temperature (especially...

15. Tips to Make the Most of a Scientific Conference

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    ...themselves and then ask questions. Practice your presentation for both instances. If your poster material ...

16. Plasmids 101: Knockout/Knock-In Plasmids

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    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

17. Google Forums Round Up: First Impressions of NgAgo

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    ...sequence of DNA. Second, NgAgo target specificity is dictated by a DNA guide sequence rather than an RNA guide...

18. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    ...method for converting heterozygous to homozygous mutations. Science aaa5945 (2015). doi:10.1126/science.aaa5945...

19. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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    ..., routine machine maintenance, and critical interpretation of the results.  If you see evidence of contamination...

20. Antibodies 101: Producing Recombinant Antibodies

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    ...Do not drop: mechanical shock in vials causes cavitation, protein aggregation, and particle formation....