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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 830 results for: eed

Showing: 821 - 830 of 830 results

  1. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...concentration of the DNA that you isolated before proceeding to your next intended step with the now gel purified...want to load on a gel, make 10% more volume than needed because several microliters can be lost in pipetting...

  3. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...Before beginning your diagnostic digest, you will need to select a restriction enzyme or enzymes that cut... vector by a single restriction enzyme, you will need to verify that it has be cloned in the correct orientation...

  4. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... which minimizes the number of small volumes you need to pipet. If you are using the same template DNA...them as a lyophilized powder. The researcher then needs to reconstitute their primers in liquid, normally...

  5. Pouring LB Agar Plates

    Type
    Protocol
    ...gel-ager mix than you think you’ll need. So for 220 mL of LB-agar, we’ll need: (37 g pre-mixed LB-agar powder...

  6. AAV ddPCR Titration

    Type
    Protocol
    ...the physical number of viral particles. Users may need to run lower or higher dilutions depending on their...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

  7. DNA Quantification

    Type
    Protocol
    ...concentration of a substance based on its absorbance, you need to know the wavelength of light that your substance...

  9. Pipetting Protocol

    Type
    Protocol
    ...pipette tips can be sterilized in an autoclave as needed to prevent contamination. When not in use, the ...
Showing: 821 - 830 of 830 results