We narrowed to 994 results for: Des

1. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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   Blog Post

   ...and workflows where CRISPR nuclease activity is desired and time or protocol steps can be saved by flexibly...

2. The Golden Stopwatch Award: Recognizing Facilitators of Research

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   Blog Post

   ...your institution’s tech transfer professionals: Describe to your TTO how unprocessed agreements slow down...

3. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

   ...activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to...

4. Fluorescent Biosensors for Measuring Autophagic Flux

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   Blog Post

   ...dynamic process into a static measurement and it provides limited information about what materials or organelles...

5. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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   Blog Post

   ...of 173 known synthetic and natural genes.  In designing and optimizing their algorithm, they found that...

6. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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   Blog Post

   ...colleagues started with the FP mNeptune684 (originally described by Li ZY, et al. PLoS One, 2016) and tested a ...

7. The Strength of Story Telling

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   Blog Post

   ...someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal forms of ...

8. New and Upcoming Viral Vectors - December 2019

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   Blog Post

   ...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...

9. Plasmids 101: TOPO Cloning

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   Blog Post

   ...needed for TOPO cloning: 1. Create Your PCR Product: Design standard primers (no need to add unique restriction...

10. Don’t Wait To Launch Your Health Science Startup

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    Blog Post

    ... own experiences founding and running miLab to describe some key lessons I learned while becoming a health...

11. Advice for Moving Into Sales After Your Science Postdoc

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    Blog Post

    ...Coordinator’ came up at Abcam. Unlike other job descriptions for marketing positions posted on recruitment...

12. Hot Plasmids: FRET-Based Biosensors

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    Blog Post

    ...beginnings of the field. Those engineered sensors were designed to replace synthetic calcium dyes, which had been...

13. Mentoring for Scientists: I Have a Mentor, What Now?

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    Blog Post

    ...download the "Mentoring for Scientists" ebook, which includes the entire series of mentoring blog posts, the...

14. Extracurricular Activities for a Strong Science Career Path

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    Blog Post

    ...how can future regulations impact your work? What design variables need consideration? Enrich your knowledge...

15. Antibody Validation Using the Indirect ELISA Method

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    Protocol

    ...This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected...Protocol Antibody Guide Introduction This protocol describes how to run an indirect Enzyme-Linked ImmunoSorbent...antigen to validate an antibody. This protocol will describe how to create a standard curve using known concentrations...antibody and an isotype control antibody to the desired concentrations in the antibody dilution buffer....HRP-conjugated isotype-specific secondary antibody to the desired concentrations in the antibody dilution buffer....Check the plate periodically to determine when the desired color change has occurred. The color will change... to optimize experimental conditions. When the desired color change has occurred, gently remove the plate...

16. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

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    Protocol

    ...Introductory techniques designed to help you get started in the lab. Name Description (Link opens in a new...gel Watch the Video! How to Design a Primer Key considerations when designing primers Watch the Video! Sequence...blocks for many more complicated procedures. Name Description (Link opens in a new window) Link to Video Making...constructing and analyzing your plasmids. Name Description (Link opens in a new window) Link to Video Restriction...titering and testing your virus preparations. Name Description Link to Video General Transfection Introduce ...Protocols for common antibody applications. Name Description Link to Video Transfection Introduce plasmid ...

17. The MTA at Addgene

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    Blog Post

    ...to note that it’s the depositing institute that decides the terms of distribution for their plasmids. ...

18. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    Blog Post

    ...ability to influence editing efficiency. In 2022, despite the increasing effectiveness of CRISPR/Cas9, scientists...

19. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...characterization of the new variants is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, ...

20. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post

    ...store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on Addgene.org ...