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  2. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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    Blog Post
    ... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

  3. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ...being targeted for autophagy. The development of several fluorescent autophagy reporters now allows for ...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule...

  6. Intro to Our Summer SciComm Series!

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    Blog Post
    ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers... have a higher level of science knowledge than average and are more likely to spend time engaging with...

  7. RUBY-Red Siliques

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    Blog Post
    ... plants (Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away...transformants can be produced. In the following generation when CRISPR needs to be removed to prevent further...

  8. 10 Great Guest Posts We're Thankful For

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    Blog Post
    ...depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested...Harvard University, describes R-bodies, “force generating protein machines that can extend and retract ...

  10. The MTA at Addgene

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    Blog Post
    ...organizations in 111 countries. From 2019 to 2023, we averaged facilitating 89,477 MTAs per year.  The MTA Process...the process, from notifications and emails to generating the UBMTA link for the recipient organization...

  11. Addgene Depositors Get More Citations

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    Blog Post
    ... plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers ...could be growing more quickly which could also generate this type of correlation. But even with that caveat...

  12. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...if in the end all they want to do is to quickly generate a single cell line and proceed to use it for experiments...researcher must amplify the library, use next-generation sequencing to ensure that the library has retained...

  13. 25 Work From Home Tips for Scientists

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    Blog Post
    ...should recommend #PhysicalDistancing. Social interactions and #social support are even more necessary ...while. Getting started early on introductions and literature reviews could make the months leading up to your...

  14. Management for Scientists: Delegating is Key

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    Blog Post
    ...effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it...of the team to ensure that no component of the overall project gets left undone or is duplicated. With...

  16. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...creating stable cell lines and are not used for general plasmid selection. Instead, reporter genes are ...material and will subsequently pass it on to future generations. Stably transfected cells may be considered an...

  17. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post
    ...(MFI) of the different populations. MFI is the average brightness from all cells that are positive for...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

  19. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...
Showing: 841 - 860 of 998 results