We narrowed to 1,002 results for: Eras

...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers... have a higher level of science knowledge than average and are more likely to spend time engaging with...

... plants (Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away...transformants can be produced. In the following generation when CRISPR needs to be removed to prevent further...

... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast... Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International Publishing...

...The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements quickly! If ...your institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal...

...being targeted for autophagy. The development of several fluorescent autophagy reporters now allows for ...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule...

...screening for binding properties. For example, several coronavirus libraries have been used to systematically... available as pooled plasmid DNA. In addition, several libraries are offered as lentiviral preparations...

...properties (percent sequence identity and percent query coverage) that could be determined through comparisons ... of engineered DNA. Both of these groups have generated robust tools that have practical applications ...

...depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested...Harvard University, describes R-bodies, “force generating protein machines that can extend and retract ...

...Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It ...Joining So you know that CRISPR can be used to generate knockouts, but how does it work? Guest contributors...

... cellular behaviors often fall short with the generation of various flux imbalances. The conventional ...authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of violacein...

... plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers ...could be growing more quickly which could also generate this type of correlation. But even with that caveat...

...if in the end all they want to do is to quickly generate a single cell line and proceed to use it for experiments...researcher must amplify the library, use next-generation sequencing to ensure that the library has retained...

...should recommend #PhysicalDistancing. Social interactions and #social support are even more necessary ...while. Getting started early on introductions and literature reviews could make the months leading up to your...

...organizations in 111 countries. From 2019 to 2023, we averaged facilitating 89,477 MTAs per year.  The MTA Process...the process, from notifications and emails to generating the UBMTA link for the recipient organization...

...effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it...of the team to ensure that no component of the overall project gets left undone or is duplicated. With...

... size of your construct. Consider buffer and temperature compatibility when digesting with more than one...due to the fact that the circular DNA takes on several conformations the most abundant being: supercoiled...

...creating stable cell lines and are not used for general plasmid selection. Instead, reporter genes are ...material and will subsequently pass it on to future generations. Stably transfected cells may be considered an...

...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...