We narrowed to 1,013 results for: Eras

...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...

..., writing won out. “I considered teaching at a liberal arts school but I’ve always enjoyed writing.” In...measure, here’s a few other posts about developing transferable skills while in grad school Resources on Addgene.org...

...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

... Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick' to introduce people all around... video where he/she/it/something is captured on camera: Unfortunately, unless they’ve worked in a lab...

...easier, cost effective and faster; this has helped accelerate scientific discoveries.” Occasionally, a plasmid...because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators worldwide...

... a learn a lot from these ASM pros and hope to leverage their expertise as we advance our mission of sharing...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries...

...aliquots could be stored indefinitely at room temperature (thus also saving freezer space).  The procedure...BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder...

...develop general technical proficiency, communicate your work, maintain ethical standards and interact professionally...

...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast... Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International Publishing...

...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers... have a higher level of science knowledge than average and are more likely to spend time engaging with...

... plants (Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away...transformants can be produced. In the following generation when CRISPR needs to be removed to prevent further...

... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

...The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements quickly! If ...your institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal...

...being targeted for autophagy. The development of several fluorescent autophagy reporters now allows for ...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule...

...place to start brainstorming our new logo. It literally underpins our DNA-derived products and symbolically...dedicated to making great science easier. Let's accelerate discovery together. Figure 10: Addgene's...

...screening for binding properties. For example, several coronavirus libraries have been used to systematically... available as pooled plasmid DNA. In addition, several libraries are offered as lentiviral preparations...

...properties (percent sequence identity and percent query coverage) that could be determined through comparisons ... of engineered DNA. Both of these groups have generated robust tools that have practical applications ...

...across several cell types is the lack of a rapid and quantitative editing assay. To help accelerate the ...

...depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested...Harvard University, describes R-bodies, “force generating protein machines that can extend and retract ...

...Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It ...Joining So you know that CRISPR can be used to generate knockouts, but how does it work? Guest contributors...