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We narrowed to 1,149 results for: Gene

Showing: 841 - 860 of 1149 results

  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...Resources on the Addgene Blog Plasmid Cloning by PCR Perform Site Directed Mutagenesis by PCR Catch up...that the exonuclease activity of the polymerase generates sticky ends in the PCR reactions. These sticky... currently an Outreach Data Curation Intern at Addgene. She is also a graduate student at the University...Background with our Plasmids 101 Posts Resources on Addgene.org Find Empty Vectors for Your Research Find Additional...

  2. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...and adenine base editors (ABEs) — can be used to generate single base pair edits without double stranded...editing systems include targeted AID-mediated mutagenesis (TAM) from the Xing Chang lab and CRISPR-X from...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource! Do you have...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

  4. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ... hear from Addgene’s former blog editor Tyler Ford on how initiative landed him at Addgene. What is initiative... Once in-person, peer-mentoring groups at Addgene have gone virtual!   2. Practice public speaking...almost every career talk I present, I’m asked what Addgene “looks for” when hiring a scientist. Of course,... comments below! Additional resources on the Addgene blog Read our science career blog posts Check ...

  5. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type
    Blog Post
    ...lab used an alternative strategy to develop a genetically encoded antibody based probe that detects the...multi-color imaging. Find the HA frankenbody tool-kit at Addgene to get started with your experiments! What else..., T., Sato, Y., … Stasevich, T. J. (2019). A genetically encoded probe for imaging nascent and mature ...PMCID: PMC6610143. Additional resources on the Addgene blog Learn about visualizing protein turnover ...fluorescent protein blog posts Resources on Addgene.org Read our fluorescent protein guide Find plasmids...

  6. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...Resources on the Addgene Blog Perform Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate... Generate Insertion and Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...the dye and the endogenous target, and thus no genetically encoded tag is required. These dyes can also ...SNAP-tag® and HaloTag®. These tags consist of a genetically encoded enzyme variant that covalently reacts... with far-red and near-infrared fluorophores. Addgene's collection contains many self-labeling protein...maintained through mechanisms like fission, fusion, biogenesis, and mitophagy, can be tracked using dyes like...Keller, P. J., Ji, N., & Lavis, L. D. (2017). A general method to fine-tune fluorophores for live-cell ...10.1016/j.bbamem.2013.09.005  More resources from Addgene Live and Let Dye: Self-Labeling Protein Tags Fluorescent...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...in setting up a FRET-based assay or screen (the Addgene blog has an excellent primer on FRET and how to...Fluorescent proteins (FPs) are the gold standard for genetically-encoded FRET donors (Fig. 1). Scores of FP variants...presented (Fig. 1) which can be carried out using Addgene plasmids. His-tag labeling reagents You may already...Gradia Lab has deposited a gateway vector with Addgene, where you can insert your protein of interest,...PubMed Central PMCID: PMC4380784. Resources on the Addgene Blog Get Tips for Using FRET in Your Experiments...Your Fluorescent Protein Basics Resources on Addgene.org Find Plasmids for FRET Browse Empty Plasmids...

  9. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...reading, you can calculate the DNA concentration. Generally, A260 of 1.0 is equivalent to 50 ug/ml pure dsDNA...samples and are often used to quantify DNA for next generation sequencing. Unlike absorbance-based methods, .... This method is often used preceding next-generation sequencing or microarray studies and is not used...suited than another. Additional resources on the Addgene blog Read more about molecular biology protocols...different grades of plasmid DNA preps Resources on Addgene.org Visit the Molecular Biology Reference Find plasmids...

  10. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...external collaborations Resources on Addgene.org Browse Addgene's COVID-19 plasmids and resources ...efforts to shift scientific culture towards a more generous and rewarding way of making science.   How...currently based in Spain and works in the field of genetics and cancer. You can follow her on Twitter at @...mahesa2504         Resources Additional resources on Addgene's blog Check out our blog post about remote collaborations...

  11. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...Fatima's work and keep checking back in with the Addgene blog for future award opportunities. Xin Zhou:...when exposed to ultraviolet light. To do so, she generated fusion proteins in which Dronpa domains flanked...Proteins, or FLiPs.  Xin later optimized these first generation FLiPs by mutating Dronpa to lower its aggregation...Chemical Biology! Additional Resources on the Addgene Blog Learn More about Photosactivatable Fluorescent...Fluorescent Protein for Your Experiments Resources on Addgene.org Find Fluorescent Protein Biosensors Browse Plasmids...

  12. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    ...specifically how dry ice can affect virus from Addgene. Bottom line: there’s good news and there’s bad... results. Order Ready to Use Viral Preps from Addgene Measuring the pH of our samples: What we did To... recommend that once you receive your AAV from Addgene, you store the AAV at -80°C for 96 hours prior ... PMCID: PMC4420252 Additional Resources on the Addgene Blog Learn How You Can Use the Viral Service Tips...Your Lentivirus Preps Additional Resources on Addgene.org Check out the Viral Service Find Virus Protocols...

  13. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... Labeling in Flow Cytometry Resource on addgene.org Addgene's Antibody Guide rAb Affinity Purification...Prathima has a research background in protein mutagenesis and cancer immunotherapies. She is currently ...Harvard Medical School.   More resources on the Addgene blog Antibodies 101: Flow Cytometry Conventional...Purification Protocol Video rAbs in the Addgene repository...

  14. Antibodies 101: ChIP

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene ...expression and protein interactions, including epigenetic modifications. But it's not an approach for the... this investment can well be worth it once you generate your data. However, it may be a good idea to add...  References and Resources More resources on Addgene’s blog Antibodies 101: Immunoprecipitation  Antibodies...

  15. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene Topics:...antibody for a…less sensitive moment.   Fig 1: Blugene fading into darkness because they accidentally ...accidentally left their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your.../help/spectral-overlap/   More resources on Addgene’s blog Antibodies 101: Monoclonal Antibodies Antibodies...

  16. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...yourself how every piece of feedback you receive generalizes.  If someone suggests a change to a slide, think... science communication, including the teams at Addgene, ComSciCon, and many more great organizations. ...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

  17. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    .... Different setting, different population, new genetic variation, or different handling can all be important... tested with a new experiment; and hypothesis generation, in which data are investigated to look for unexpected...trends or differences between groups. Hypothesis generation is critical for advancement, but we are too often...using hypothesis testing statistical tools that generate a p-value.  As an example, if you hypothesize ...clear distinction between hypothesis testing and generation, can be sure that results will be reported regardless...

  18. Chemogenetics Plasmids

    Type
    Collection
    ...AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Service: Chemogenetics AAV Blog: Chemogenetics Viral Vectors 101 Blog: Chemogenetics vs. Optogenetics Chemogenetics...Collections Chemogenetics Plasmids Chemogenetics Plasmid Collection You may also like... Chemogenetics Guide Viral...biological processes. Addgene's collection contains two main classes of chemogenetic receptors: Designer... a bimodal opto-chemogenetic actuator. For more detailed descriptions of chemogenetic receptors, ligands...your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search ...Find plasmids for chemogenetic experiments, including popular DREADDs and PSAMs. Plasmid...

  19. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ... can be lot-to-lot variability in activity. No Genetic Tagging - Since antibodies are produced in animals...instead of via recombinant DNA, they can’t be genetically tagged and instead are conjugated to reporters...monoclonal antibodies, but they are laborious to generate and costly to share. What is a RANbody? A RANbody...PubMed PMID: 29440485. Additional Resources on the Addgene Blog Learn more about using the secondary nanobody...Check out this post. Additional Resources on Addgene.org More nanobody expression plasmids can be found...
Showing: 841 - 860 of 1149 results