We narrowed to 856 results for: HA

1. Fluorescent Proteins 101: When GFP lets you down

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   Blog Post

   ...deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum around 488 nm and it emits ...

2. CRISPR-mediated Plant Base Editors

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   Blog Post

   ...editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need to convert an ...

3. Transferable Skills Guide: Conflict Resolution

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   ...organization, because confirming that every person has read and understood every communication that gets...

4. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...

5. Antibodies 101: Beyond Surface Labeling

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   ...selecting the primary antibody host species, as it has to be different from the other antibodies in your...

6. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...where the vector that you want to use is very large, has a high GC content, contains a lot of repeats--any...

7. Plasmids 101: Common Lab E. coli Strains

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   ...OpenWetWare's E.coli genotype resource. Additionally, NEB has a great list of genetic markers for your reference...

8. Finding Your Science Policy Path

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   ...ever thanks to the virtual world that the pandemic has foisted upon us. Action Items:  A simple place to...

9. Treating Muscular Dystrophy with CRISPR Gene Editing

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   ...any immunogenicity of the AAV vector Once delivery has been optimized, it’s important to ascertain how long...

10. Antibodies 101: Introduction to Antibodies

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    ...methods. Each type of antibody and production strategy has advantages and disadvantages compared to each other...

11. Antibodies 101: Designing Your First Flow Panel

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    ...few commonly used fluorophores. Each fluorophore has a defined emission maximum. Note, you can also use...

12. Live and Let Dye: Self-Labeling Protein Tags

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    ... These tags most often consist of an enzyme that has been mutated to block its catalytic activity and ...

13. CRISPR 101: Multiplex Expression of gRNAs

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    ...multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use mammalian...

14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

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    Blog Post

    ...high contrast ratio usually indicates that a PA-FP has a low level of spontaneous fluorescence in the absence...

15. Trimmer Lab NeuroMab Collection

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    Collection

    ...collection . Protocols The UC Davis/NIH NeuroMab Facility has several protocols available that may be helpful for...

16. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ...your gel or blot, Table 1 (at the end of the post) has a number of helpful suggestions.  Gels Gel issues...