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We narrowed to 873 results for: NSI

Showing: 841 - 860 of 873 results

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Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

Type

Blog Post

...think plant biology is massively underfunded considering how important it is. I would really like to reach...
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Type

Blog Post

...multi-spacer CRISPR array. Workflow and technical considerations Making insertions in bacterial genomes with...
Cpf1: A New Tool for CRISPR Genome Editing

Type

Blog Post

...the target sequence (bases 19-23). Cpf1 is also sensitive to single mismatches, but variably so, with Kleinstiver...
To Codon Optimize or Not: That is the Question

Type

Blog Post

...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page ...
Overcoming the AAV Size Limitation for CRISPR Delivery

Type

Blog Post

...streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display...
The PAM Requirement and Expanding CRISPR Beyond SpCas9

Type

Blog Post

...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...
Plasmids 101: Broad Host Range Plasmids

Type

Blog Post

...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...
Rett Syndrome: A History of Research and Therapeutic Outlooks

Type

Blog Post

...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...
Plasmids 101: Codon usage bias

Type

Blog Post

...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...
Using Video to Share Your Science: We Share Science

Type

Blog Post

...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

... and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

...unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...
Transferable Skills Guide: Time Management

Type

Blog Post

.... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture...
Thoughts on the Future of Research 2015 Boston Symposium

Type

Blog Post

...different universities are trying to compile more comprehensive demographic information about the postdoc work...
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

Type

Blog Post

...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...
CRISPR 101: Epigenetics and Editing the Epigenome

Type

Blog Post

...cells. For example, KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced...
Technique: Probe Phage Genomes for Host Binding Proteins

Type

Blog Post

...transformants for appropriately sized inserts. We consider a library worth using if at least 40% of colonies...
Plasmids 101: Restriction Cloning

Type

Blog Post

...stains): Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...
Building Global Connections with the International Mentorship Program USA-EUROPE

Type

Blog Post

...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the...
A History of Genome Engineering in Popular Culture

Type

Blog Post

... scientific community and remind us to always consider the ethics and broader implications of our research...

Showing: 841 - 860 of 873 results

Results per page:

Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

Cpf1: A New Tool for CRISPR Genome Editing

To Codon Optimize or Not: That is the Question

Overcoming the AAV Size Limitation for CRISPR Delivery

The PAM Requirement and Expanding CRISPR Beyond SpCas9

Plasmids 101: Broad Host Range Plasmids

Rett Syndrome: A History of Research and Therapeutic Outlooks

Plasmids 101: Codon usage bias

Using Video to Share Your Science: We Share Science

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Harnessing Bacterial Toxins for Allelic Exchange

Transferable Skills Guide: Time Management

Thoughts on the Future of Research 2015 Boston Symposium

Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

CRISPR 101: Epigenetics and Editing the Epigenome

Technique: Probe Phage Genomes for Host Binding Proteins

Plasmids 101: Restriction Cloning

Building Global Connections with the International Mentorship Program USA-EUROPE

A History of Genome Engineering in Popular Culture

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