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  2. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...consistent levels of Cas9 expression between experiments and over time (as compared to separate transductions...

  3. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...have to weed through fewer colonies after your experiments. As a common example, a selection will leave ...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

  4. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    ... Unlike traditional dye-based neuronal tracing reagents, AAV particles require receptor-mediated entry...neurons. When using this serotype to conduct experiments with chemogenetic vectors, this property must...

  5. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...diversity in Vector Assisted Spectral Tracing (VAST) experiments using PHP.eB in the brain give some insight into...Xinhong Chen, and Miggy Chuapoco, and to all our participants for their time and excellent questions.    ...

  6. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...validation pipeline. (B) Schematic shows the separate elements of the R-mAb expression plasmid involved in coexpression...R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue to be available...

  7. Hot Plasmids - February 2022

    Type
    Blog Post
    ... is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a has generally been underutilized...low-pressure teaching opportunity for lab members or students new to protein expression and purification. ...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    ...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...switches to producing IgE antibodies, the gene segments for IgM, IgD, and IgG are deleted.     ...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... how to use viruses to set up these systems. Components of a biosensor Some of the biosensors used in...transducer will generate a numerical value which represents the amount of analyte present in the system at...

  10. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...just getting started using antibodies in your experiments, you may be curious about all of the different... are first injected with a small peptide that represents a short, unique amino acid sequence found in ...

  11. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ... into the key takeaway themes and customer pain points we’ve identified. I can’t wait to send it out. ...work on next to help alleviate any customer pain points. Many thanks to our guest blogger Rachel Rubinstein...

  15. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

  19. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

  20. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...
Showing: 841 - 860 of 1015 results