We narrowed to 949 results for: ache
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TypeBlog Post...undergraduates and PhD students so that they can help support each other and provide advice. The society organizes ...
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CRISPR/Cas9 FAQs Answered!
TypeBlog Post...genotyping (using Sanger sequencing, for example) on each colony in order to screen for positive ones. -... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...339 (6121): 819–23. Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. ... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...constant regions. Variable regions are unique to each antibody and convey specificity to the target while... -
What the HEK?
TypeBlog Post...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo... -
Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog Post...features like serotype, promoter, or tool type and each entry contains an image along with key experimental... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...While the various AAV serotypes can be used to achieve different patterns of expression, researchers often... -
Fluorescent Biosensors for Measuring Autophagic Flux
TypeBlog Post...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...addition GFP tags are large, dim, and permanently attached to a protein, making them hard to amplify and ... -
Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science
TypeBlog Post...away from the loaded and judgemental language. In each peer-review, I try to mention what I liked about... -
Single-cell tracking of lineage and identity with CellTag
TypeBlog Post...acts as a unique identifier for individual cells. Each cell is labeled with 3-4 tags to increase the diversity... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...Florida State University, they used a mutagenesis approach and found that specific amino acid changes favored... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...knowledge and passion on display in our building, each day brushing past and chatting with individuals ... -
Intro to Our Summer SciComm Series!
TypeBlog Post... Rachel Leeson... -
RUBY-Red Siliques
TypeBlog Post...the segregation of the CRISPR construct could be achieved much easier than by PCR or a fluorescent microscope... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post... a signal as with the wild-type AraC, it still reached the level of expression needed for most applications... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog Post...reporter (in this case a fluorophore) directly attached to it. (B) Another solution is to combine your... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...rechargeable battery, is relatively small, and can be detached easily whenever necessary. This is all an impressive... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features...