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We narrowed to 869 results for: lic

Showing: 841 - 860 of 869 results

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein composition. It is used for a variety of applications such as measuring target protein expression ...

  12. Cloning

    Type
    Guide
    ...example of a LIC protocol on our website. Ligation Independent Cloning (Figure adapted from LIC protocol ... repaired by the normal replication process. It is important to note that LIC has difficulty assembling...Independent Cloning Ligation Independent Cloning (LIC) relies on the 3'-5' exonuclease activity of T4 DNA...removes nucleotides from the end of a DNA strand. In LIC, the T4 DNA polymerase’s exonuclease activity creates...are repaired by the bacteria after transformation. LIC does not require site-specific recombination or a...rapid cloning method. So how does this work exactly? LIC depends on the addition of only one free dNTP to ...interest and then insert them into plasmids for replication and protein expression. So how do scientists ...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...potential formation of replication-competent viruses. While AAV themselves are replication incompetent, there...production of replication-competent viruses that would be able to infect cells and replicate autonomously... actively causing symptoms or replicating. Lytic cycle Viral replication process where the virus uses ...into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and reproduce.... The Rep region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which...which are involved in genome replication and viral packaging. The Cap region encodes three structural ...virus. The ITR sequences serve as origins of replication and also act as signals for packaging. Figure...

  14. Molecular Biology Reference

    Type
    Guide
    ... Origin of Replication ( ori ) DNA sequence which directs initiation of plasmid replication (by bacteria...double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. Although they...the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter cell receives...manipulating genes. For instance, most plasmids are replicated in E. coli and are relatively small (∼3000 - ...sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a ... exists extrachromosomally in E. coli , it is replicated independently and segregated to the resulting...form, plasmids require a bacterial origin of replication ( ori ), an antibiotic-resistance gene, and at...

  15. Adenovirus Guide

    Type
    Guide
    ... DNA replication and host cell manipulation. Late transcripts are expressed after DNA replication starts...production of replication-competent adenoviruses (RCAs) that would be able to infect cells and replicate autonomously...26–45 kb in length and contain multiple heavily-spliced transcripts flanked by two inverted terminal repeats..., which has limited their use in therapeutic applications. However, this has also made them the most widely...presence of adenoviral genes E1, E4, E2a and VA for replication. For more information about AAVs, read our AAV...whereas the E1 region is essential for viral replication and therefore needs to be supplied through other...due to their well-established methods, relative simplicity of production, and higher titers. However, the...

  16. Antibody Guide

    Type
    Guide
    ...single assay. Overview of Antibody Applications Antibody-based applications can be generally classed into ...these applications, visit the Antibody section of the Addgene Protocols page. Antibody Applications - Quantification...production and storage techniques, and explain common applications. Science... Handling Visualization Signal Amplification Application Overview Quantification Methods Capture Methods...of other antibodies. This has useful research applications, and is covered in the following Isotypes section...signaling molecules and are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments... molecules that can be distinguished in your application. The indirect detection method uses an unconjugated...

  17. CRISPR Guide

    Type
    Guide
    ...Experiment Web References PAM Sequences Glossary Publications CRISPR Overview Bacteria have an interesting... every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became the most... to various Cas enzymes have extended CRISPR applications to increasingly complex functions, including...understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR... Cas12a, which are available for specialized applications. Check out our list of additional Cas proteins...genomic edits carried out by Cas9. Multiplexing applications include editing multiple genes at once; using...to help you select an optimized gRNA for your application (see: Plan Your Experiment ). In addition to ...

  18. Lentiviral Vector Guide

    Type
    Guide
    ... Rev Response Element (RRE) within unspliced and partially spliced transcripts to facilitate nuclear export...and tropism. Replication-competent The ability of a virus (or viral vector) to replicate and reproduce...hosts in order to continue their lifecycle and replicate. This feature can be harnessed for stable delivery...of safety improvements and the potential for replication-competent lentivirus production — that is, creation...particles that could infect cells and further replicate on their own. While the viral components are still...Interchangeable; usually encodes for VSV-G Safety Safe; replication incompetent by using three separate plasmids ...plasmids encoding the necessary viral genes Safer; replication incompetent by using four plasmids instead of ...

  19. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...and tropism. Replication-competent The ability of a virus (or viral vector) to replicate and reproduce...hosts in order to continue their lifecycle and replicate. This feature can be harnessed for stable delivery... for safety to reduce the chance of creating replication-competent retroviruses. For a summary of all ...retroviral vectors are commonly used in gene therapy applications to more specifically direct delivery of gene...vectors are a popular choice in gene therapy applications. There are many approved gamma-retroviral gene...cell (CAR-T) therapy is a particularly popular application of gamma-retroviral vectors. Gamma-retroviral...vectors are: The potential for generation of replication-competent gamma-retroviral vectors The potential...

  20. Modular Cloning Guide

    Type
    Guide
    ...a variety of applications—like building synthetic genetic circuits, engineering metabolic pathways, assembling...Modular Cloning Blog: Plasmids 101 Modular Cloning Applications and Kits Blog: Plasmids 101 Golden Gate Cloning...editing, functional genomics, and more complex metabolic engineering and synthetic biology, compatible ...proteins for use in E. coli for a variety of applications. EcoFlex MoClo Extension, Volume I Bacterial... vectors for use in E. coli for a variety of applications. CIDAR MoClo Parts Kit Bacterial Expression ...toolkit with both high cloning capacity and vector simplicity, compatible with other MoClo, GoldenBraid, and...multi-part assemblies in either integrative or self-replicating plasmid vectors for working in cyanobacteria....
Showing: 841 - 860 of 869 results