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We narrowed to 876 results for: lic

Showing: 841 - 860 of 876 results

  3. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...understood, but some possible reasons include: Metabolic pressures - it takes cellular resources to produce...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ... similar to humans. For example, due to their simplicity, yeast have become an important experimental ...

  15. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...protein composition. It is used for a variety of applications such as measuring target protein expression ...

  19. Molecular Cloning Techniques

    Type
    Guide
    ...Independent Cloning (LIC) relies on the 3' to 5' exonuclease activity of T4 DNA polymerase. In LIC, the T4 DNA ...where the normal replication process will repair the nicks. It is important to note that LIC has difficulty... structures. Read more in our SLIC blog post . Figure 6: Summary of LIC. Created with BioRender.com. Yeast-mediated...nicks that bacteria repair after transformation. LIC does not require site-specific recombination or a...making it an easy, cheap, and rapid cloning method. LIC depends on the addition of only one free dNTP to ...interest and then insert them into plasmids for replication and protein expression. There are many methods... 101: Sequence and Ligation Independent Cloning (SLIC) More Plasmids 101 topics More Plasmids cloning ...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...potential formation of replication-competent viruses. While AAV themselves are replication incompetent, there...production of replication-competent viruses that would be able to infect cells and replicate autonomously... actively causing symptoms or replicating. Lytic cycle Viral replication process where the virus uses ...into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and reproduce.... The Rep region encodes four non-structural replication proteins (Rep78, Rep68, Rep52, and Rep40), which...which are involved in genome replication and viral packaging. The Cap region encodes three structural ...virus. The ITR sequences serve as origins of replication and also act as signals for packaging. Figure...
Showing: 841 - 860 of 876 results