We narrowed to 850 results for: met

1. General Transfection

   Type

   Protocol

   ... Introduction This protocol describes a general method for transfecting mammalian cells using linear polyethylenimine...

2. Protocol - Bacterial Transformation

   Type

   Protocol

   ...your transformation procedure is working. TIP: Sometimes less is more. Although it may be counter-intuitive...

3. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...digestion . Most restriction enzymes digest DNA asymmetrically across their recognition sequence, which results...

4. Lentivirus Production

   Type

   Protocol

   ...incubator Pipet controller Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high ...

5. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ...; or c) loading less DNA into the well. Another method for visualizing very short DNA fragments is polyacrylamide...

6. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...bacterial proteins and genomic DNA. Depending on the method used, the DNA concentration and purity will vary...

7. What is Polymerase Chain Reaction (PCR)

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   Protocol

   ... Polymerase Chain Reaction (PCR) is an in vitro method that allows a single, short region of a DNA molecule...

8. AAV Titration by qPCR Using SYBR Green Technology

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   Protocol

   ...inverted terminal repeat sequences. Hum Gene Ther Methods. 2012 Feb;23(1):18-28. (Link opens in a new window...

9. Pouring LB Agar Plates

   Type

   Protocol

   ...right temperature, we recommend using a laser thermometer. Light the flame at the plate pouring station...

10. Plasmid Cloning by PCR (with Protocols)

    Type

    Protocol

    ...calculate this based on DNA concentration alone. One method is to conduct 2 ligations for each plasmid you ...