We narrowed to 903 results for: nes

1. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   Blog Post

   ... via plasmids or by the creation of stable cell lines and these cells are then grown in biotin-containing...

2. Lab Automation at Addgene

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   Blog Post

   ...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...

3. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   ...exceptions to our standard workflow.  Table 1: Guidelines for the data fields in the deposit spreadsheet...

4. 6 Tips for Grant Writing

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   Blog Post

   ...something that made them better than my unsuccessful ones. With grant applications there’s going to be an ...

5. Tips for Technical Support Calls

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   Blog Post

   ... Abcam and NEB have Ph.D.s answering emails and phones). We can’t be experts on every technique or experiment...

6. Plasmids 101: FLEx Vectors

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   Blog Post

   ...orientation, and type of target sites that flox your genes of interest, a powerful FLEx switch can be created...

7. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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   Blog Post

   ...the expected complete sequence for the lacI/lacZ genes (1), while sequencing of white colonies showed a...

8. PhD Applications After COVID

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   Blog Post

   ...interview or campus visit in person, things like sickness or scheduling issues can and do interfere with...

9. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems for genome engineering...

10. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...McCawley, P. (1977). The establishment of two cell lines from the insectspodoptera frugiperda (lepidoptera...

11. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...photobleaching, lack of red-shifted indicators, and brightness of fluorescence all have room for improvement...

12. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...

13. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ...BioMolecular Modeling, we spun off a small, family-run business – 3D Molecular Designs – to commercialize some...

14. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...

15. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...Coloc 2, BioImageXD, and customized CellProfiler pipelines. Image analysis software produces quantifiable...

16. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

17. Experimenting with New Careers while in Grad School

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    Blog Post

    ...science outreach: engaging the public to promote an awareness and understanding of science. Like most science...

18. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and...

19. Droplet Digital PCR for AAV Quantitation

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    Blog Post

    ...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...

20. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper...