We narrowed to 1,012 results for: RING
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TypeBlog Post...ideal with Myc, as low pH conditions are required during the purification, which may affect protein function...
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Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...This instability can cause partial loss of an ITR during routine plasmid propagation, with complete ITR ... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post...stable frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672... -
Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi
TypeBlog Post...changes favored blue-shifting more than others. During this process, they identified mWasabi, a bright... -
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants
TypeBlog Post...accelerate ongoing COVID-19 research. “I hope that by sharing the plasmids other scientists would be able to ... -
Plasmids 101: TOPO Cloning
TypeBlog Post...electro-competent E. coli - excess salt causes arcing during electroporation which would cause the electroporation... -
Plasmid Cloning by PCR
TypeBlog Post...of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Preparing the PCR product for cloning Run the PCR reaction... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog Post...edition eBook include: new chapters on genome engineering, cloning, and plasmid tags, addtional content... -
Another Pathway into Cells: iTOP
TypeBlog Post... Mary Gearing... -
Chemogenetics Guide
TypeGuide...through the Gαq/11 G-protein and activate neuronal firing through stimulating phospholipase C, which releases...muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D Rat M3 muscarinic ...adrenergic G αs CNO* Increase cAMP Neuronal burst firing Guettier et al., 2009 hM4Di hM2Di Human M4 muscarinic...and Sternson SM (2011). Chemical and genetic engineering of selective ion channel-ligand interactions....Moore CI, Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies...Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM,... -
Multiplexed Overexpression of Regulatory Factors (MORF) collection
TypeBlog Post...differentiation. Fig. 2: Single cell RNA-seq clustering of cells transduced with the TF pooled library... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog Post...biology, cell biology, microbiology, genetic engineering and strategic knowledge to solve a clinical disease... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...Expanding the targeting range of CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog Post... track the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased ... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...stability and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius... -
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids
TypeBlog Post...thanks to Daniela Bourges for editing this post and sharing her technical expertise in persistent identifiers... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...all these reasons, the QCRG is delighted to be partnering with Addgene, who will be taking over the distribution... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post... most often completed your habit is useful for figuring out when to schedule times to write. Pomodoro... -
Pushing the Limits of DNA Assembly
TypeBlog Post...sgRNA for CRISPR experiments can be laborious, requiring multiple cloning steps. You can simplify this ...