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We narrowed to 1,009 results for: Eras

Showing: 861 - 880 of 1009 results
  1. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    ...should recommend #PhysicalDistancing. Social interactions and #social support are even more necessary ...while. Getting started early on introductions and literature reviews could make the months leading up to your...
  2. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...if in the end all they want to do is to quickly generate a single cell line and proceed to use it for experiments...researcher must amplify the library, use next-generation sequencing to ensure that the library has retained...
  3. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it...of the team to ensure that no component of the overall project gets left undone or is duplicated. With...
  4. Plasmids 101: Mammalian Vectors

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    Blog Post
    ...creating stable cell lines and are not used for general plasmid selection. Instead, reporter genes are ...material and will subsequently pass it on to future generations. Stably transfected cells may be considered an...
  5. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post
    ...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...
  6. AAV Q&A with Tim Miles

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    Blog Post
    ...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...
  7. Antibodies 101: Isotypes

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    Blog Post
    ... of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the...in their heavy chain constant regions result in several unique physical characteristics of these five isotypes...
  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

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    Blog Post
    ...(MFI) of the different populations. MFI is the average brightness from all cells that are positive for...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...
  9. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...
  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post
    ...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...
  11. Plasmids 101: Yeast Vectors

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    Blog Post
    ...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...
  12. Human Germline Editing Using CRISPR

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    Blog Post
    ...human embryos has been met with a storm of media coverage. But the paper published August 2nd in Nature ...present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define whether...
  13. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...
  14. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...  Prime editing improvements By Angela Holmes Several labs have recently shared some exciting plasmid...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...
  15. Addgene's Tips for Plasmid Quality Control

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    Blog Post
    ...plasmids, such as special growth instructions (temperature, strain, media supplements), suggested sequencing...re like us, you need a strategy for processing several sequencing reactions in parallel. We have a few...
Showing: 861 - 880 of 1009 results