We narrowed to 898 results for: NSI

...fine-tuning dosage so that enough wild-type MECP2 is made inside the cells, but this is exciting progress in tackling...

...Molecular Biology Reference Read about genetic code expansion Browse our plasmid collection page  ...

...streptavidin and next-generation sequencing). Using this sensitive method, they found that SaCas9 did not display...

...Wolfe SA, Sontheimer EJ (2018) NmeCas9 is an intrinsically high-fidelity genome-editing platform. Genome...

...the labeled regions, as well as the strength and density of labeling in each. This allows users to quickly...

...plant and animal systems. Importantly, they allow transient delivery, enabling transgene-free genome editing...

...2008), but there are still many more features to consider when designing a full gene. A non-exhaustive list...

...host range recombinant vectors Replicons, which consist of a plasmid’s ori and all of its control elements...

...Evolution of Scientific Impact Data Freedom: The Expansion of Data Sharing in Research Publications Resources...

... and model systems. We have used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes...

.... For instance, even if I had planned my day extensively, my lack of desire to go sit in the tissue culture...

...unmarked mutants, but it frequently requires considerable optimization to ensure strong counter-selection...

...different universities are trying to compile more comprehensive demographic information about the postdoc work...

...cells. For example, KRAB induced silencing was transient and quickly reversed in culture. However, DNMT3A-induced...

...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

...transformants for appropriately sized inserts. We consider a library worth using if at least 40% of colonies...

...stains):  Stain Pre or Post Run? Visualization Sensitivity (ng DNA) SYBR Safe Pre and Post Blue or UV ...

...possible a significant qualitative and quantitative expansion in the 2015-2016 academic year. This year, the...

... scientific community and  remind us to always consider the ethics and broader implications of our research...

...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...