We narrowed to 1,022 results for: NTS

1. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...control in many genetic targeting and editing experiments. Find the CASANOVA plasmids here! Bubeck F et...for protein-protein interaction (PPI) studies in plants that rely on transient transfection systems using...

2. Celebrating 15 Years of Scientific Sharing

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   ...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own ...scientists who request from us but also by high school students and industry scientists as a universal resource...

3. New Tool for Lineage Tracing: The ClonTracer Library

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   ...not cancer cell populations resistant to drug treatments arise during treatment or are preexisting in ...really great way of broadly sharing these kinds of reagents.” Here at Addgene, we’re excited to see the creative...

4. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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   ...control spatially as to what’s happening at different points within the wood. There are different types of cellulose...hundreds of genes. We’ve created enough regulatory elements, CRISPRs, repressors, activators. We’ve been putting...

5. Adenoviral Vector Production and Troubleshooting

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   ...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

6. Rabies and Neuronal Tracing

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   ... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...

7. Open Resources and Plasmid Tools For Studying C. elegans

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   ... best strategies and protocols for different experiments.  In this blog post let's take a look at some...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

8. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...

9. Lab Automation at Addgene

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   ...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...under BSL1 conditions.  Patents or Licenses If there are any patents or licenses that could prohibit...

11. Antibodies 101: Choosing the Right Isotype

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    ...-species staining protocols that use antibody fragments to block the endogenous immunoglobulins and reduce...complex with a variety of proteins interacting. Experiments tend to be equally complex with users trying ...

12. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    ...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

13. Gaining Leadership Skills Volunteering at a Professional Organization

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    ...format depending on the particular style and requirements of the new Board Members. In some instances ...when it is so important for postdocs and graduate students to think about their career options, gaining transferable...

14. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...when trying to perform high-throughput experiments. If a user wants to carry out a high-throughput genetic...

15. Cloning Mammalian Cells with the Agarose Method

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    ...heat- the inside of the wrist or the cheek. (Parents of infants will have no problem with this!) Make sure...

16. Tips to Make the Most of a Scientific Conference

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    ...the conference Review your favorite conference moments on social media and if there was something you ... with people you don’t know or attending social events. I like to set goals for myself like meet ten new...

17. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...essence, pluripotent cells can be thought of as descendants of totipotent cells. An example of pluripotent...that are produced during in vitro fertilization treatments and donated afterwards for ESC research purposes...

18. Plasmids 101: Knockout/Knock-In Plasmids

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    ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...participating in development or other physiopathological events to which the chosen promoter responds. You can ...

19. Viral Vectors 101: The Retroviral Lifecycle

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    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein... Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors...

20. CRISPR Antimicrobials

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    ...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...