We narrowed to 1,082 results for: TOR;

1. Antibodies 101: Epitope Tags

   Type

   Blog Post

   ...was originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well as a ...

2. Antibodies 101: Epitope Availability

   Type

   Blog Post

   ... understand, to the best of your ability, what factors may be affecting the protein’s configuration in...

3. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   ...to gain entry into human cells via human ACE2 receptors. In their assay, the pseudoviruses express a luciferase...

4. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

   Type

   Blog Post

   ...May 13, 2021 The original AraC transcriptional regulator Their system is based on the AraC protein that...

5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

   Type

   Blog Post

   ...advantageous if you’re working with small capacity viral vectors such as AAV. Base removal RNA-guided CRISPR enzymes...

6. Donations from Addgene to Yield Answers for Rare Disease Researchers

   Type

   Blog Post

   ...rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying four...

7. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

   Type

   Blog Post

   ... arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve...

8. Another Pathway into Cells: iTOP

   Type

   Blog Post

   ...limitations of packaging size associated with viral vectors. For these reasons, iTOP, especially in tandem ...

9. 25 Work From Home Tips for Scientists

   Type

   Blog Post

   ...radio reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare ...

10. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type

    Blog Post

    ...to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence...

11. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type

    Blog Post

    ...turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in ...

12. Summer SciComm Series: A PhD in Science Communication

    Type

    Blog Post

    ...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...

13. How to Lead a Great Meeting

    Type

    Blog Post

    ... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ...

14. Components of CRISPR/Cas9

    Type

    Blog Post

    ... to modify the desired genomic locus.   Some historical notes CRISPR arrays in bacterial genomes consist...

15. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    ...Purification Protocol Video rAbs in the Addgene repository...

16. CasPEDIA: A Functional Classification of Cas Enzymes

    Type

    Blog Post

    ... any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme...

17. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    ... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for...

18. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ...secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there...

19. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type

    Blog Post

    ...excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature...

20. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    ...these strains should not be used for long term storage. Additional Resources Review of restriction modification...