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...knowledge and passion on display in our building, each day brushing past and chatting with individuals ...

...  Rachel Leeson...

...the segregation of the CRISPR construct could be achieved much easier than by PCR or a fluorescent microscope...

... a signal as with the wild-type AraC, it still reached the level of expression needed for most applications...

...addition GFP tags are large, dim, and permanently attached to a protein, making them hard to amplify and ...

...away from the loaded and judgemental language. In each peer-review, I try to mention what I liked about...

...acts as a unique identifier for individual cells. Each cell is labeled with 3-4 tags to increase the diversity...

...Florida State University, they used a mutagenesis approach and found that specific amino acid changes favored...

...on counting the number of autophagosomes. This approach, however, has inherent limitations because it ...

...challenging, with protocols using dsDNA templates achieving ~1-10% efficiency. Easi-CRISPR uses a long ssDNA...

...response to customer feedback. An example of this approach is SnapGene’s algorithm for detecting common features...

...reporter (in this case a fluorophore) directly attached to it. (B) Another solution is to combine your...

...rechargeable battery, is relatively small, and can be detached easily whenever necessary. This is all an impressive...

...constant regions. Variable regions are unique to each antibody and convey specificity to the target while...

...transfect the ‘helper’ plasmids (viral packaging machinery) along with the viral plasmid containing the cargo...

...While the various AAV serotypes can be used to achieve different patterns of expression, researchers often...

...1658796. PubMed Central PMCID: PMC52876. 2. Novel approach to molecular cloning and polynucleotide synthesis...

...staggered (Cpf1) cleavage. NgAgo takes a different approach, randomly removing 1-20 nucleotides from the cleavage...

...is no coming back. The whole lab is emotionally attached to this disease.” He says that the award from ...

... readouts.” Griesbeck also points out that the approach he and his colleagues took to optimize sensitivities...