Skip to main content
Addgene
Showing: 861 - 880 of 1073 results
  1. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...There must be some way to use LEGOs, which are practically universally loved (except for those rare times...children who have no preconceived obstacles, optimistically grasp at limitless potential, and who sometimes...Research 5 Reasons to Use Reddit for Science Communication 10 Ways to Share Your Science Resources on ...
  2. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    ...control over timing. Because chemogenetics relies on chemical activation and the diffusion of drugs throughout...beneficial for use in therapeutic or behavioral applications where drawn out effects are required. Targeted...require a permanent intracranial implant to be surgically installed to allow for light delivery. Chemogenetics...
  3. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...let curiosity guide your intellectual journey. Practical approaches One approach to achieve this goal ...chemistry, even if your primary focus lies within a biological lab. Engaging in such seminars exposes you to...ourselves moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking...
  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...determination, don’t generally affect antibody applications like ELISAs or Western blots, they are key in... (ADCD) Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent...antibodies on a pathogen activates the immune system's classical complement pathway, starting a cascade of events...
  5. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...not silenced.    Find CellTag libraries here!  Applications of CellTag The Morris Lab developed CellTag ...(CellTagD13), which is when the iEP phenotype typically emerges. Every three days, a sample of cells was...conversion, but CellTag could be used to study other biological processes where cells are undergoing identity...
  6. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...serotypes are equal; while some serotypes are typically high yielding, for others, such as AAV2, low yields...AAV yields. Given the wide range of downstream applications scientists rely on our AAV preparations for,... of adeno-associated virus/phage." Journal of Biological Chemistry (2012): jbc-M112. PubMed PMID: 22915587...
  7. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...community about how the antibodies work in various applications, enabling you to better plan your experiments...the target antigen, and performance in various applications are included in the full webpage. Educational...recombinant antibody production as well as antibody applications. In addition, basic and advanced affinity reagents...
  8. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... that will bring about the next revolution in biological research. We’re incredibly happy to see that ... colonies to find a successful clone? Addgenie Jessica Welch explains how Blue-White screening could be...repository use Cas9 to cut DNA, Addgene Science Communications Intern Mary Gearing explains how Cas9 homolog...
  9. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success...
  10. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...while most of the world was locked down, a South African company wanted to test if a type of antibodies ...-and-error. A recommendation for the correct statistical test can save a retraction. Today, collaborations...postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the coordinators...
  11. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...cleaving sequences which are similar, but not identical, to their defined recognition sequence. Most enzyme... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols...
  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...powered plasmid maps far easier to navigate. These practical and effective changes will give you the ability...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...feature names, locations, sizes, colors used to indicate them on the map, direction (if relevant), and ...
  13. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ... specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...
  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...transfer plasmid sequences into the host genome. Typically, it is the sequences between and including the...pseudotyping.   Figure 1: Genome of a prototypical gamma-retrovirus.    Frequently asked questions... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...
  15. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...cDNA, which  was used as a template for PCR amplification with a degenerate primer set to amplify IgG ...  While screening by IF-ICC worked well for identification of the correct clones, it was determined that...through functional assays. Following functional verification, the R-mAb expression plasmids were DNA sequenced...
  16. Antibodies 101: Isotypes

    Type
    Blog Post
    ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...chain constant regions result in several unique physical characteristics of these five isotypes, such as...their heavy chain constant domains. These are typically numbered, i.e. IgG1, IgG2, etc. Let’s dive in ...
  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ..., partial words, phrases, partial phrases, or numerical identifiers. We also combine the different terms... higher than matches of the same name in the publication field. Suggestions We provide as-you-type suggestions...PI labs and institutions. There are many more technical details that make our new search engine unique...
Showing: 861 - 880 of 1073 results