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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 921 results for: ire;

Showing: 861 - 880 of 921 results

  5. Plasmids 101: Protein tags

    Type
    Blog Post
    ...cytometry, but rarely used for purification as elution requires low pH poly His HHHHHH  0.8 Affinity and Purification...

  18. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...Sharps container Biohazard waste container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before...eyewash station, safety shower, fire blanket, and extinguisher are also required to be present in the room....each level, as each level has different safety requirements. BSL-1 is designated for those working with ...times when you may be working with a protocol that requires shaking or mixing, which may produce aerosols ... PPE protocols . Working in a BSL-2 laboratory requires laboratory glasses in addition to coat and gloves...room and on the door. BSL-2 centrifugation steps require an aerosol-tight lid and rotors should be loaded...in a biosafety cabinet. BSL-2 safety protocols require bloodborne pathogens training. It is strongly recommended...

  19. Western Blot

    Type
    Protocol
    ...stringent buffers and a sonication step may be required for hard to extract proteins such as those in ...standard curve. Determine the volume of sample required to load an equivalent amount of total protein ...continue running the gel until you have obtained the desired separation. Gently open the gel with the spatula...Device. Close the lid of the device. Select the desired method and make sure the parameters are correct... incubation Dilute the primary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...peroxidase-conjugated secondary antibody to the desired concentration in blocking buffer. Pro-Tip The optimal...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ... as bone, or bacteria/yeast/plant samples will require additional steps to effectively lyse open the cells...wash and add RNase-free water or TE as soon as the entire tube is dried but while the white pellet is still...Pro-Tip To avoid multiple freeze-thaw cycles of your entire RNA sample, consider making smaller aliquots of...
Showing: 861 - 880 of 921 results