We narrowed to 903 results for: nes

1. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...

2. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction...

3. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...

4. The Importance of a Fun Workplace: Company Culture at Addgene

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   ...place to work, we do SO many fun things!!”  Okay - honestly, maybe not everyone replies with quite so much...

5. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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   ...you like to bring your phone in the lab, though, honestly, this still grosses me out. Maybe it’s just me...

6. Plasmids 101: Plasmid Incompatibility

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   ...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...

7. Antibodies 101: Immunoprecipitation

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   ... Common negative controls include knockout cell lines or tissues that do not express the protein. With...

8. Hot Plasmids - August 2020

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   ...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

9. Hot Plasmids - November 2023

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   ...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...

10. Viral Production at Addgene

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    ... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...

11. How to Be an Excellent Trainee

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    ...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

12. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...

13. Fluorescent Proteins 101: Aptamer Fluorophores

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    ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

14. Changing Labor Laws Bring Increased Postdoc Wages

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    ...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...

15. 5 Reasons to Use Reddit for Science Communication

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    ...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...

16. Important Considerations in Optogenetics Behavioral Experiments

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    ...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...

17. Plasmids 101: E. coli Strains for Protein Expression

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    ...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...

18. Antibodies 101: Introduction to Gating in Flow Cytometry

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    ...make stricter gates while some like to make looser ones (Figure 3). A more “liberal” gate in the beginning...

19. Using Phosphoserine to Study Protein Phosphorylation

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    ...must be recoded to ‘TGA’ using site-directed mutagenesis. In the C321.ΔA strain, this TGA will be recognized...

20. Thoughts on the Future of Research 2015 Boston Symposium

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    ... At Addgene we try to do our part in boosting awareness of various career opportunities through our career...