We narrowed to 906 results for: nes

1. Tips for Using BLAST to Verify Plasmids

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   Blog Post

   ...website to verify plasmids or validating your new clones, try these tips to make the most of your time and...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...Cas9M1C/C80S) that harbors two surface exposed cysteines, including the native C547, allowing for ligation...

3. AAVs CREATed for Gene Delivery to the CNS and PNS

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   ... new capsids (i.e. the part of the virus that determines tropism) have been developed that increase transduction...

4. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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   ... of the mean rate for each paper. Green and red lines depict the sum of mean +1 and -1 std for each paper...

5. Droplet Digital PCR for AAV Quantitation

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   ...parameters influencing droplet rain in the Listeria monocytogenes prfA assay-reduction of ambiguous results in...

6. Tips from the Repository Trenches: Using Barcodes to Track Samples

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   ...transforms newly deposited samples from Monday through Wednesday. Each sample that is transformed that week is ...

7. The Importance of a Fun Workplace: Company Culture at Addgene

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   ...place to work, we do SO many fun things!!”  Okay - honestly, maybe not everyone replies with quite so much...

8. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

9. Hot Plasmids - August 2020

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   ...consisting of CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

10. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...

11. Antibodies 101: Immunoprecipitation

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    ... Common negative controls include knockout cell lines or tissues that do not express the protein. With...

12. Hot Plasmids - November 2023

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    ...Through Envelope Design). This modular approach combines an interchangeable set of fusogenic and targeting...

13. Viral Production at Addgene

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    Blog Post

    ... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...

14. How to Be an Excellent Trainee

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    Blog Post

    ...mindful that your mentor will still have their own deadlines and priorities to attend to. Being mindful looks...

15. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    Blog Post

    ... your gene to be in relation to the rest of the genes? And do you want it to be in the same (forward) ...

16. Plasmids 101: Plasmid Incompatibility

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    ...pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes & Genetic Systems 77:1–9 . https://doi.org/10.1266...

17. Changing Labor Laws Bring Increased Postdoc Wages

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    ...Yourself for a New Career in Science with Our Career Readiness Posts Learn Good Management for Scientists Resources...

18. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...You can check out the r/Science AMA Submission Guidelines here, but essentially we just needed to send ...

19. Important Considerations in Optogenetics Behavioral Experiments

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    ...Witten, Ilana B., et al. "Recombinase-driver rat lines: tools, techniques, and optogenetic application ...

20. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...below or browse Addgene’s collection of empty backbones.   Our Most Popular Plasmids 101 Posts: Origin...