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Showing: 861 - 880 of 1015 results

  1. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...phenotype and helps improve the reproducibility of experiments. Inbred mice are particularly useful for understanding...recessive traits and their impact on diseases or treatments, but are not useful for genetic engineering. ...

  2. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...Antibodies are used in many different experiments that require scientists to detect proteins in their...visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference being...

  4. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...more complex and the presence of helper virus contaminants have slowed the use of this system. New and ...purification of rAdV uses cesium chloride (CsCl) density gradients combined with ultracentrifugation to separate ...

  5. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... al., 2013). The study found that at early time points, most of the projections to these new neurons came...from nearby neurons. It wasn’t until later time points that longer-range connections were seen. This study...

  6. Lab Automation at Addgene

    Type
    Blog Post
    ...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

  8. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...the conference Review your favorite conference moments on social media and if there was something you ... with people you don’t know or attending social events. I like to set goals for myself like meet ten new...

  9. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...participating in development or other physiopathological events to which the chosen promoter responds. You can ...

  13. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Because it is made at the same time as the other components of the vector particle, some of the RT protein... Addgene blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors...

  14. CRISPR Antimicrobials

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    Blog Post
    ...While challenges remain in the delivery of these agents, CRISPR antimicrobials could become our newest ... both favors antibiotic sensitive bacteria and prevents sensitive bacteria from acquiring resistance genes...

  15. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...and me. To that end, here are my personal 10 commandments for making it all work – most of the time. #1...transportation, call a cab and remember their own school assignments, the better. In my opinion, a level of independence...

  16. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...with binding, and some common antibody buffer components can interfere with conjugates. Make sure to do...

  17. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...Usage of a pooled library lentiviral prep for experiments. Pooled library lentiviral preps are generally...for sequencing, representative NGS results (read counts or another visualization) that confirm diversity...

  18. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...it leaves little room for additional regulatory elements. Feng Zhang's group previously packaged Cas9 and...Resources on the Addgene Blog Learn more about Cas9 Variants and the Expanded CRISPR Toolbox Read about CRISPR...

  19. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...mechanism which exclusively yields non-crossover events. This means all newly synthesized sequences are...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...
Showing: 861 - 880 of 1015 results