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We narrowed to 971 results for: lars;

Showing: 441 - 450 of 971 results

  1. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...the conference you are attending on the larger side? At large conferences, chances are that several talks... the other person seemed confused, bored, or particularly excited about? If you tried tweaking your pitch... contact information if your conversation is particularly fruitful. After the conference Bring back what...LinkedIn or Twitter, for instance. If you’ve had a particularly meaningful conversation, and you have their ...

  2. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...pathways and represent the largest family of human drug targets. To enable large-scale profiling of many ...then used single-molecule FRET to study the intramolecular dynamics of the proteins in solution and when...by endogenous GPCR. Example applications include large-scale pharmacological profiling or interrogation...conditions, revealing context-dependent responses and molecular patterns of selectivity. With 10 plasmids available...

  3. Pooled Library Amplifications

    Type
    Blog Post
    ... prone to recombination, while a library with a large backbone might be more difficult to transform.  ... per transformation may vary depending on the particular type of cells you are using, as well as other...generally needs to be determined experimentally. For larger libraries that require hundreds of nanograms of...full-size plasmids (or for smaller plasmids to overtake larger plasmids, if the library varies significantly in...looking at protocols used for libraries that are similar in size and composition as examples. Table 1 provides...

  4. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...also possess similar morphologies, growth properties, and express stem cell markers similar to ESCs [11...lentiviral infections respectively [9, 10]. These stellar works led to a Nobel Prize for Dr. Yamanaka in ...11].  While iPSCs and ESCs have similar functional utility, iPSCs bypass the transplantation compatibility...2. Jaenisch, R. and R. Young, Stem cells, the molecular circuitry of pluripotency and nuclear reprogramming...

  5. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...Medicine. She is particularly interested in science communication and fundamental molecular biology.      ... it. About three or four years into my PhD in Molecular Biology, I realized I wanted a career in science...experience taught me. The article submitted was hilarious, full of interesting information, and received...researchers. It is an excellent way to meet people with similar interests! Especially as someone interested in ...

  6. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...the same cassette is sometimes advantageous, particularly when only a portion of the plasmid is packaged...discovered - many from viruses, but also some from cellular mRNAs. What they all have in common is the ability...have some disadvantages. These elements are quite large (500-600 bp) and may take up precious space in viral... are short (about 20 amino acids) and produce equimolar levels of mulitple genes from the same mRNA. The...posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information. ...

  7. Deep Dive: qPCR

    Type
    Blog Post
    ...actions to a single step. Scorpion chemistry in particular has been reported to work well in the Lightcycler...specifically aimed at determining the best options for a particular cell or tissue type (such as here, here, or here...be worth the initial effort of setting it up for larger or frequently analyzed sample types and sets.   ...analogous Wilcoxon tests have all been shown to give similar results, so you only need to pick your favorite...Real-Time PCR: Methods and Protocols, Methods in Molecular Biology, vol. 1160, DOI 10.1007/978-1-4939-0733... S; Huggett J. qPCR primer design revisited. Biomolecular Detection and Quantification. Volume 14. 2017...

  8. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...tools for efficient and programmable insertions of large DNA cargos into bacterial genomes. This new capability...Type II systems (such as CRISPR-Cas9). Unlike in regular Type I CRISPR-Cas systems, however, Cascade from...V-K CRISPR-transposon systems function in a very similar manner to Type I-F, but instead employ a Cas12k...CRISPR-transposons excel at programmable insertions of even very large genetic cargo, at one or multiple desired genomic...systems have already been demonstrated, ranging from large genomic insertions and/or deletions, to multiplexed...

  9. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...and injection methods are best suited to their particular scientific question - even when others have already...to successfully replicate published works. Of particular importance to these efforts is the open sharing...Reports are reviewed by Addgene scientists for clarity and accuracy, and by the end of the year, those...Chemically and Functionally Distinct Vectors. Molecular Therapy - Methods and Clinical Development, 18...occurrence during vector purification and formulation. Molecular Therapy, 12(1), 171–178. https://doi.org/10.1016...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...other antibiotics listed below affect continuous cellular processes (e.g., protein synthesis), while amp...synthesis as their mode of action and have a very similar relationship as amp and carb (they are interchangeable...all bacteria used in research, it isn’t a widely popular selection agent. Pros: Stable (spectinomycin). .../1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance to the antibiotic Zeocin...gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-Agar Antibiotic Plating...
Showing: 441 - 450 of 971 results