We narrowed to 1,013 results for: CAL

...should have identical sequences on the ends (sequences A and B in the figures). These identical sequences...PCR with primers that contain a 5′ end that is identical to an adjacent segment and a 3′ end that anneals..., Hutchison CA, Venter JC, Merryman C. (2010). Chemical synthesis of the mouse mitochondrial genome. Nature...

...make bacteria more susceptible to either chemical or electrical based transformation, generating what are...antibiotic containing agar plate. This step is not critical for Ampicillin resistance but is much more important...large plasmids (>10 kb) or BACs, what can I do? Chemically competent cells are fast and easy to use, but...

...visualization and purification. Electrophoresis uses an electrical field to move the negatively charged DNA through...eye protection and gloves when working with this chemical. Note: If you add EtBr to your gel, you will also...approximately 75-80% of the way down the gel. A typical run time is about 1-1.5 hours, depending on the...polyacrylamide gel electrophoresis (PAGE), which is typically used to separate 5 - 500 bp fragments. How do ...

...medium. This dot is composed of millions of genetically identical bacteria that arose from a single bacterium...draw in discontinuous lines. Start by streaking a vertical line of bacteria along one edge of the plate. ...

...polymerase can create overhangs of varying length (typically 10-12 bp) based on a specific sequence. The annealed... the replication cycle. Empty vectors for LIC typically employ type II restriction enzymes (e.g. BsaI)...back the guanosine residue and become stalled. A typical T4 Pol reaction is shown. Mix the components (...

...lab! Introduction Microscopes are emblematic of biological research and are found in many different types...suggests, light microscopes take advantage of the physical properties of light to detect small objects. Two...the light follows from the source to your eyes is called the light path . Light travels from the light source...

... watching, and we hope you found the video radd-ically helpful!    More resources at Addgene Addgene'...

...share quick tips to help you master fundamental technical lab skills, like pipetting! We’ll release a new...

...edition of our Viral Vectors 101 eBook!  This practical guide to using viral vectors in the lab is a helpful...

...Mackin Lab from University of Colorado -Anschutz Medical Campus (@CSD_CUAMC) caught a case of holiday cheer...

...your life a little easier. These can range from technical tips as in our episode on Barcoding, to basic ...

... depositing paper for more information Edward Callaway   For researchers in countries with applicable...

...software engineers, surprisingly, who have deep technical knowledge but little biology experience.) The ...

...leading a professional development workshop. Specifically, Jo, Brook, and Angela are co-leading "Making...

...open platform for scientists using AAV to share practical information about their experiences. It will contain...

...on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders DNA ...

...Rob Hurt, whose expertise and suggestions were critical to the development and writing of two blog posts...

...demethylation. AAV Q&A with Tim Miles: While not technically a research story, I couldn't resist adding in...

...a technology is so useful it forever enhances biological research and discovery. CRISPR is a great example...

...Addgene.  Learn how discoveries were made and get practical tips on how to use the plasmids in your own lab...