We narrowed to 1,012 results for: RING

1. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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   Blog Post

   ...PubMed PMID: 30639098. LipoGlo: A reporter for measuring ApoB bound lipoproteins Article contributed by...

2. 5 Great Apps for Lab Life

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   Blog Post

   ...research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that ...

3. Components of CRISPR/Cas9

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   Blog Post

   ... endonuclease function, when used for genome engineering, the only CRISPR protein required is the Cas9...

4. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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   Blog Post

   ...here's a screenshot from the Chroma Spectra Viewer comparing a standard Cy3 or Rhodamine filter set (Chroma...

5. Lentiviral Vector Guide

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   Guide

   ...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the gamma-retrovirus MMLV...LEDGF (also known as PSIP1 or p75 ), a lentiviral tethering gene that helps facilitate integration. Despite...markers, such as the puromycin resistance gene, conferring antibiotic resistance to infected host cells....target. Lentiviral vectors are a common way of delivering these gRNAs, due to their stable expression and...libraries popular for lineage tracing experiments and monitoring the dynamics of heterogeneous tumors. Browse ...References Cronin, J., Zhang, X., & Reiser, J. (2005). Altering the Tropism of Lentiviral Vectors through Pseudotyping...

6. Communicating Your Science With Help From ComSciCon

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   Blog Post

   ...audience your primary focus When writing a paper or preparing a presentation, it's easy to forget that you have...

7. Plasmids 101: Screening Strategies Used in Plasmid Cloning

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   Blog Post

   ... Addgene, we now use next-generation sequencing during our QC process. This way, we can confirm the sequence...

8. Building and Validating Recombinant Antibodies: A Case Study

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   Blog Post

   ...reproducibility. The Trimmer Lab found that in addition to recovering RNA from hybridomas grown in tissue culture, ...

9. Sweating the Small Stuff: Details in the Lab

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   Blog Post

   ...double-checking details, or just taking a moment to relax. During really stressful days, I would even take advantage...

10. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post

    ... alternative antimicrobial.  Conjugates And HRP brings us handily to the final topic of this post, the...

11. Improved Plasmid Maps Powered by SnapGene

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    Blog Post

    ...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...

12. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    Blog Post

    ...brightness of two orange fluorescent proteins, differing by only a single amino acid. Without further information...

13. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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    Blog Post

    ...would need to be generalizable and capable of measuring a variety of molecular signatures or cancer phenotypes...

14. Multiplex Genome Editing with CRISPR-Cpf1

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    Blog Post

    ...Cpf1 vs. Cas9, see the table below. Table 1. Comparing the Cas9 and Cpf1 CRISPR Nucleases   Cas9 Cpf1...

15. Screening for Successful Genome Editing with Digital PCR

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    Blog Post

    ...of NHEJ to generate translational reading frame-altering indels for “functional knockout” of a gene of ...

16. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...2016, we launched our Viral Service and began delivering ready-to-use lentivirus and adeno-associated ...

17. CasPEDIA: A Functional Classification of Cas Enzymes

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    Blog Post

    ...? CasPEDIA operates like many search engines, featuring both a general search bar tool and an advanced...

18. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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    Blog Post

    ...because they are only able to enter the nucleus during mitotic breakdown of the nuclear envelope. Lentiviruses...

19. Viral Vectors 101: Viral Applications

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    Blog Post

    ...host cell (the target of viral infection) and delivering a genetic payload. This cargo is typically their...

20. Behind the scenes: Addgene’s new search engine and more

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    Blog Post

    ...materials in our catalog are very well-characterized. During this time, we’ve also created a variety of educational...