We narrowed to 1,052 results for: FER

1. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

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   ...al. applied TRIM-away to nine proteins in ten ddifferent cell types, including difficult-to-manipulate...that TRIM21 overexpression did not affect cell proliferation. These cells also did not contain changes in...

2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ... invasive to use optogenetics in the brain.  References Bedbrook CN, Yang KK, Robinson JE, Mackey ED,...Read more about optogenetics Learn about the differences between chemogenetics and optogenetics Resources...

3. The time and cost required to make a plasmid

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   ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...period. For ease of calculations*, let’s split the difference and put scientific inflation at… roughly 34.9%...

4. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   ...us develop these resources further. Feel free to offer your comments and suggestions, and even to submit...submit a guest blog entry of your own! References 1. Bernard, P. “Positive Selection of Recombinant DNA ...

5. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   ... plasmid libraries containing combinations of different gRNAs in each plasmid. Each gRNA combination can...systems. He can be contacted at [email protected].   References 1. Cheng, Allen A., Huiming Ding, and Timothy...

6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...and industry jobs. Since there are a variety of different career paths that don’t all need another degree...-op. It has been so insightful to learn about different career paths, successes and failures, values, ...

7. Early Career Researcher Toolbox: Finding Relevant Papers

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   ...virtual bookshelf. Pro tip The “cited by” and references sections of a PubMed entry are great places to...This library can also be synced with a Mendeley reference manager account. Pro tip Sign up for weekly email...

8. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   ... to address the unique needs of students from different departments across the university and alterations...public policy. Follow David on Twitter @driglar.   References: 1. Alberts, Bruce, et al. "Rescuing US biomedical...

9. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   ...academic labs. Being unable to publish or attend conferences makes it very challenging to compete in today...pressure to publish and more freedom to attend conferences, but after receiving her degree she was faced...

10. 3D Printing Meets CRISPR Cas9

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    ...explaining CRISPR technology to 4th graders, check out Jennifer Doudna’s new book A Crack in Creation. Making... the target DNA strand, and then activate two different endonuclease active sites. Fortunately, there ...

11. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...species, like S. aureus and N. meningitidis require different PAM sites than SpCas9, in this case, NNGRRT and...is working for you in the comments section! References Hu, Johnny H., et al. "Evolved Cas9 variants ...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...treatment (Stephens et al., 2019). If sufficient gene transfer and knock-in is achieved, producer cells could...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...

13. Academic vs. Industry Postdocs

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    ... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...LinkedIn connections and other contacts to get that referral and find out more about the company’s program....

14. Plasmids 101: Knockout/Knock-In Plasmids

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    ...against improper, random recombination into a different locus. Correctly recombined cells will not contain...should increase. New genome engineering tools also offer hope for the creation of new knockout animal models...

15. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...human genome-wide CRISPRko (knock out), CRISPRi (interference), and CRISRPa (activation) libraries that do...conditions such as model systems and primary cells. References 1. Doench, John G., et al. "Rational design of...

16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

17. SciComm: Taking Science to Elementary and Middle Schools

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    ...expertise with our district middle school was a very different experience since, unlike elementary schools, middle...such a valuable extension to our curriculum and offered engaging examples of how understanding genetics...

18. Summer SciComm Series: Modes of Communication

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    ...truth, people are incredibly varied in how they prefer to consume information. Personally, I would much...outcome to the limitations and potential of your preferred platform before you start can save a lot of trouble...

19. CRISPR Antimicrobials

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    ...mixed population, represented here by cells of different colors, specifically targets bacteria with the...ground in the fight against bacterial superbugs. References 1. Citorik, Robert J., Mimee, Mark, and Timothy...

20. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...Joanne’s back yard this year, though she generously offered. Instead we picniced outside of our new office ...Netflix series. The thing that has made all the difference here at Addgene is the emphasis placed on team...