We narrowed to 937 results for: KIN

1. Troubleshooting Your Plasmid Cloning Experiment

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   Blog Post

   ...verify that the sequence of the plasmid you are working with is correct. It's also best to use 1-2 ug of...

2. Antibodies 101: Secondary Antibodies

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   Blog Post

   ... good deal of it. Researchers are continuously looking for ways to improve direct methods and avoid the...

3. Viral Vectors 101: An Introduction to AAV

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   Blog Post

   ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

4. To Each HIS Own

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   Blog Post

   ...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient...

5. Antibodies 101: Polyclonal Antibodies

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   Blog Post

   ...some regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased...

6. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

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   Blog Post

   ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

7. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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   Blog Post

   ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

8. Addgene's Tips for Plasmid Quality Control

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   Blog Post

   ... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what...

9. Plasmids 101: Cre-lox

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   Blog Post

   ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

10. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step...

11. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    Blog Post

    ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

12. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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    Blog Post

    ...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

13. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...

14. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...

15. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

16. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want...

17. CRISPR in the Clinic

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    Blog Post

    ...pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ...

18. A Look at Addgene's QC Process

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    Blog Post

    ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...

19. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

20. Plasmids 101: Terminators and PolyA signals

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    Blog Post

    ...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation...