We narrowed to 936 results for: Kin
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TypeBlog Post...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...
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Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ... -
To Each HIS Own
TypeBlog Post...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations... -
Addgene's Tips for Plasmid Quality Control
TypeBlog Post... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what... -
Plasmids 101: Cre-lox
TypeBlog Post...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want... -
CRISPR in the Clinic
TypeBlog Post...pipelines (viral), and researchers are currently working on giving this treatment a CRISPR spin. CRISPR ... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody... -
A Look at Addgene's QC Process
TypeBlog Post...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...eBook to get all the background you need to start working with plasmids! What are termination and polyadenylation... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog Post...membrane-bound spike protein, in a remarkable 12 working days. While the lab used this method for COVID-...